Corin R E, Haspel H C, Peretz A M, Sonenberg M, Rifkind R A
Cancer Res. 1986 Mar;46(3):1136-41.
Butyrate, at concentrations greater than 0.75 mM, induces hemoglobin accumulation in murine erythroleukemia cells (MELC). At concentrations below 0.75 mM, butyrate inhibits hemoglobin accumulation induced by hexamethylene bisacetamide (HMBA) as well as HMBA induced commitment to terminal cell division. The blocking of HMBA induced differentiation does not result from growth inhibition. When cells were exposed to HMBA and butyrate for 4 days and then both inducers were removed, the cells did not terminally divide. On the other hand, cells exposed to HMBA for 4 days, with subsequent removal of HMBA, did go on to terminally divide. Thus, butyrate blocks the ability of HMBA to accumulate the intracellular signals for terminal cell division. A 48-h pretreatment of cells with butyrate did not inhibit the ability of subsequent HMBA treatment, after butyrate removal, to induce terminal cell division. These results might suggest that cells do not generate a memory of exposure to HMBA in the presence of butyrate or a memory of exposure to butyrate when used as a pretreatment under these conditions. HMBA, at concentrations below 1.0 mM, does not induce MELC differentiation, but such concentrations actually enhance dimethyl sulfoxide induced differentiation of MELC. Equimolar concentrations of short chain fatty acids (1 to 7 carbons) were tested for their ability to block HMBA induced differentiation of MELC. Butyrate and valerate (4 and 5 carbons, respectively) had blocking activities similar to each other, whereas the other fatty acids exhibited little or no blocking of HMBA induced differentiation.
浓度大于0.75 mM的丁酸盐可诱导小鼠红白血病细胞（MELC）中血红蛋白的积累。在浓度低于0.75 mM时，丁酸盐会抑制由六亚甲基双乙酰胺（HMBA）诱导的血红蛋白积累以及HMBA诱导的终末细胞分裂。对HMBA诱导的分化的阻断并非由生长抑制导致。当细胞暴露于HMBA和丁酸盐4天，然后去除这两种诱导剂时，细胞不会进行终末分裂。另一方面，暴露于HMBA 4天，随后去除HMBA的细胞则会进行终末分裂。因此，丁酸盐阻断了HMBA积累终末细胞分裂细胞内信号的能力。用丁酸盐对细胞进行48小时预处理，在去除丁酸盐后，并不抑制随后HMBA处理诱导终末细胞分裂的能力。这些结果可能表明，在存在丁酸盐的情况下，细胞不会产生对暴露于HMBA的记忆，或者在这些条件下用作预处理时，不会产生对暴露于丁酸盐的记忆。浓度低于1.0 mM的HMBA不会诱导MELC分化，但这种浓度实际上会增强二甲亚砜诱导的MELC分化。测试了等摩尔浓度的短链脂肪酸（1至7个碳）阻断HMBA诱导MELC分化的能力。丁酸盐和戊酸盐（分别含4和5个碳）具有相似的阻断活性，而其他脂肪酸对HMBA诱导的分化几乎没有或没有阻断作用。
