Maurer W, Braendle W
Horm Metab Res. 1979 Jan;11(1):60-3.
After in vivo application of [125 I]-iodo-hCG to pseudopregnant rats, radioactivity extracted from ovarian particulate fractions can be eluted from Sephadex G--200 columns in three various fractions (Vo, Kav = 0.15, Kav = 0.27) differing from hCG (Kav = 0.35). By lowering the pH 50% of the radioactivity is precipitated and the resuspended material shows a conversion to the smaller complexes. The supernatant material is dissociated to [125I]-iodo-hCG. In alkaline milieu [125I]-iodo-HCG is dissociated completely from the complexes.
将[125I]-碘代人绒毛膜促性腺激素应用于假孕大鼠体内后，从卵巢颗粒部分提取的放射性物质可从葡聚糖凝胶G - 200柱上以三种不同组分（Vo、Kav = 0.15、Kav = 0.27）洗脱下来，这与hCG（Kav = 0.35）不同。通过降低pH值，50%的放射性物质沉淀，重新悬浮的物质显示出向较小复合物的转化。上清液物质解离为[125I]-碘代人绒毛膜促性腺激素。在碱性环境中，[125I]-碘代人绒毛膜促性腺激素从复合物中完全解离。
