Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; Department of Microbiology, Weifang Medical University, Weifang 261053, China.
J Virol Methods. 2021 Dec;298:114298. doi: 10.1016/j.jviromet.2021.114298. Epub 2021 Sep 22.
Enterovirus A (EV-A) species are the main agents responsible for hand, foot, and mouth disease (HFMD), a serious public health concern. Lack of appropriate reagents prevents the mechanistic study of these virus infections. 2C protein, a non-structural protein of Enterovirus, is crucial for viral replication and antiviral immunity. Here, preparation and testing of a monoclonal antibody by immunizing mice with Coxsackievirus A10 protein 2C (CVA10-2C) was reported. This antibody could identify most EV-A types, both conventional and unconventional groups. We also mapped the antibody epitope SLATGIIARA, which is highly conserved in EV-A species and located in the ATPase domain. Some key amino acids include G140, I141, I142, and R144. In conclusion, we generated a recombinant monoclonal antibody against multiple EVA types and confirmed its performance, which may facilitate the future study of Enterovirus A infection and many potential applications, such as the diagnosis of pathogen or the development of antiviral therapies.
肠道病毒 A（EV-A）是引起手足口病（HFMD）的主要病原体，手足口病是一种严重的公共卫生问题。缺乏适当的试剂阻碍了对这些病毒感染的机制研究。肠道病毒的非结构蛋白 2C 蛋白对于病毒复制和抗病毒免疫至关重要。本研究通过用柯萨奇病毒 A10 蛋白 2C（CVA10-2C）免疫小鼠来制备和测试单克隆抗体。该抗体可识别大多数 EV-A 型，包括常规和非常规组。我们还对抗体表位 SLATGIIARA 进行了定位，该表位在 EV-A 种中高度保守，位于 ATP 酶结构域中。一些关键的氨基酸包括 G140、I141、I142 和 R144。总之，我们生成了针对多种 EVA 型的重组单克隆抗体，并验证了其性能，这可能有助于未来对肠道病毒 A 感染的研究和许多潜在应用，如病原体诊断或抗病毒疗法的开发。
