MarR-type transcription factor RosR regulates glutamate metabolism network and promotes accumulation of L-glutamate in Corynebacterium glutamicum G01.

Transcription factors (TFs) perform a crucial function in the regulation of amino acids biosynthesis. Here, TFs involved in L-glutamate biosynthesis in Corynebacterium glutamicum were investigated. Compared to transcriptomic results of C. glutamicum 13032, 7 TFs regulated to glutamate biosynthesis were indentifed in G01 and E01. Among them, RosR was demonstrated to regulate L-glutamate metabolic network by binding to the promoters of glnA, pqo, ilvB, ilvN, ilvC, ldhA, odhA, dstr1, fas, argJ, ak and pta. Overexpression of RosR in G01 resulted in significantly decreased by-products yield and improved L-glutamate titer (130.6 g/L) and yield (0.541 g/g from glucose) in fed-batch fermentation. This study demonstrated the L-glutamate production improved by the expression of TFs in C. glutamicum, which provided a good reference for the transcriptional regulation engineering of strains for amino acid biosynthesis and suggested further metabolic engineering of C. glutamicum for L-glutamate production.