Ripka J, Stanley P
Somat Cell Mol Genet. 1986 Jan;12(1):51-62. doi: 10.1007/BF01560727.
The cytotoxic plant lectins from P. sativum have been used to select new lectin-resistant mutants from Chinese hamster ovary (CHO) cells. Two novel phenotypes that behave recessively but fall into the same complementation group have been termed Lec13 and Lec13A. Both of these mutant types are phenotypically reverted to pea lectin sensitivity following growth in L-fucose. In contrast, two other unique phenotypes behave dominantly in somatic cell hybrids and maintain their pea lectin-resistance in the presence of L-fucose. They have been termed LEC14 and LEC18, respectively. The lectin-resistance and complementation properties of the four new mutant types suggest that they define three different glycosylation genes of the CHO genome.
来自豌豆的细胞毒性植物凝集素已被用于从中国仓鼠卵巢（CHO）细胞中筛选新的凝集素抗性突变体。两种表现为隐性但属于同一互补群的新表型被命名为Lec13和Lec13A。在L-岩藻糖中生长后，这两种突变类型在表型上都恢复为对豌豆凝集素敏感。相比之下，另外两种独特的表型在体细胞杂种中表现为显性，并且在存在L-岩藻糖的情况下保持其对豌豆凝集素的抗性。它们分别被命名为LEC14和LEC18。这四种新突变类型的凝集素抗性和互补特性表明它们定义了CHO基因组的三个不同糖基化基因。
