Rogers Amber N, Mastronardo Maya K, Mekonnen Tsion G, Soto Ana Maria
Molecular Biology, Biochemistry & Bioinformatics Program, Towson University, Towson, MD, 21252, USA.
Department of Biological Sciences, Towson University, Towson, MD, 21252, USA.
Anal Biochem. 2021 Nov 1;632:114391. doi: 10.1016/j.ab.2021.114391. Epub 2021 Sep 22.
RNA represents a vibrant area of research and many studies use techniques that require large amounts of purified RNA. One common purification method involves slicing a section of a polyacrylamide gel containing the RNA of interest and eluting the RNA out of the gel using electroelution. Various electroeluter models are available but sometimes a given model becomes discontinued, compelling researchers to choose a different model. Here, we have compared two electroeluters with different chamber designs for their ability to recover RNA from gel pieces. Our results show that both electroeluters are effective and recover comparable amounts of purified RNA.
RNA代表了一个充满活力的研究领域,许多研究使用的技术需要大量纯化的RNA。一种常见的纯化方法是切割含有目标RNA的聚丙烯酰胺凝胶切片,并使用电洗脱法将RNA从凝胶中洗脱出来。有各种不同的电洗脱仪型号可供选择,但有时某一特定型号会停产,这迫使研究人员选择不同的型号。在这里,我们比较了两种具有不同腔室设计的电洗脱仪从凝胶块中回收RNA的能力。我们的结果表明,这两种电洗脱仪都有效,并且回收的纯化RNA量相当。