一种用于从聚丙烯酰胺凝胶中高浓度制备分离蛋白质的创新性环形电洗脱器。

An innovative ring-shaped electroeluter for high concentration preparative isolation of protein from polyacrylamide gel.

作者信息

Chen Jing-Jing, Li Guo-Qing, Pratush Amit, Jahan Sharmin, Kong Fan-Zhi, Xiao Hua, Fan Liu-Yin, Cao Cheng-Xi

机构信息

Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

出版信息

Anal Biochem. 2017 Apr 15;523:39-43. doi: 10.1016/j.ab.2017.01.023. Epub 2017 Jan 27.

DOI:10.1016/j.ab.2017.01.023

PMID:28137604

Abstract

A ring-shaped electroeluter (RSE) was designed for protein recovery from polyacrylamide gel matrix. The RSE was designed in such a way that a ring-shaped well was used to place gel slices and an enrichment well was used to collect eluted protein samples. With HSA as model protein, the electroelution time was less than 30 min with 80% recovery rate, and the concentration of recovered protein was 50 times higher than that of conventional method. The RSE could be reused at least ten times. The developed device makes great advance towards economic electroelution of biomolecules (such as proteins) from gel matrix.

摘要

设计了一种环形电洗脱仪（RSE）用于从聚丙烯酰胺凝胶基质中回收蛋白质。RSE的设计方式是，用一个环形孔放置凝胶切片，用一个富集孔收集洗脱的蛋白质样品。以人血清白蛋白（HSA）作为模型蛋白，电洗脱时间少于30分钟，回收率达80%，回收蛋白的浓度比传统方法高50倍。RSE至少可重复使用十次。所开发的装置在从凝胶基质中经济地电洗脱生物分子（如蛋白质）方面取得了很大进展。