雷公藤甲素对人胶质瘤细胞放射敏感性的影响及其机制

Effects of triptolide on radiosensitivity of human glioma cells and its mechanism.

作者信息

Shu Mengting, Li Chenguang, Zhong Chen, Huang Tongyi, Wang Xinzhuang, Tan Yang, Zhao Wenyang, Xie Xiaoyan

机构信息

Department of Medical Ultrasonics, Division of Interventional Ultrasound, Institute of Diagnostic and Interventional Ultrasound, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

出版信息

J BUON. 2021 Jul-Aug;26(4):1549-1555.

PMID:34565018

Abstract

PURPOSE

To study the effect of triptolide (TP) on radiosensitivity of human glioma U251 cells and its mechanism, so as to provide new ideas and methods for the radiotherapy of glioma.

METHODS

U251 cells were treated with 10, 50, 100 nmol/L TP at different concentrations and irradiated with 0, 2, 4, 6, 8 Gy X-ray. The radiosensitivity of cells in each group were detected by MTT. U251 cells were then divided into control group, 10 nmol/L TP group, 4 Gy radiation group, 10 nmol/L TP + 4 Gy radiation group. The formation ability of U251 cells in each group was detected by colony formation assay. Flow cytometry was used to detect cell cycle and apoptosis in each group. Western blot was used to detect the changes of PI3K/Akt signal pathway in each group.

RESULTS

When 10, 50, 100 nmol/L TP were combined with 2, 4, 6, 8 Gy X-ray, the proliferation inhibition rate of U251 cells in each group increased significantly (p<0.05); compared with 10 nmol/L TP alone group and 4 Gy radiation alone group, the colony formation ability rate of U251 cells in 10 nmol/L TP + 4Gy radiation combined group decreased significantly (p<0.05), the cell cycle was blocked in G1 phase, and the apoptosis rate was significantly reduced (p<0.05). The level of p-pi3k and p-Akt decreased significantly (p<0.05).

CONCLUSION

Triptolide could significantly increase the radiosensitivity of human glioma U251 cells and play a role by inhibiting the PI3K/Akt signal pathway.

摘要

目的

研究雷公藤甲素（TP）对人胶质瘤U251细胞放射敏感性的影响及其机制，为胶质瘤的放射治疗提供新的思路和方法。

方法

用10、50、100 nmol/L不同浓度的TP处理U251细胞，并分别给予0、2、4、6、8 Gy X射线照射。采用MTT法检测各组细胞的放射敏感性。将U251细胞分为对照组、10 nmol/L TP组、4 Gy照射组、10 nmol/L TP + 4 Gy照射组。采用集落形成实验检测各组U251细胞的形成能力。采用流式细胞术检测各组细胞周期及凋亡情况。采用蛋白质免疫印迹法检测各组PI3K/Akt信号通路的变化。

结果

当10、50、100 nmol/L TP与2、4、6、8 Gy X射线联合作用时，各组U251细胞的增殖抑制率均显著升高（p<0.05）；与单独10 nmol/L TP组和单独4 Gy照射组相比，10 nmol/L TP + 4 Gy照射联合组U251细胞的集落形成能力率显著降低（p<0.05），细胞周期阻滞于G1期，凋亡率显著升高（p<0.05）。p-pi3k和p-Akt水平显著降低（p<0.05）。