Acta Virol. 2021;65(3):303-306. doi: 10.4149/av_2021_305.
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically significant pathogens in swine industry of China. To study infection and genetic variation of PRRSV, 637 tissue samples were collected from diseased pigs in Shandong, and then subjected to detection of PRRSV. The nsp2 and ORF5 genes were sequenced for investigation of variations and phylogenetic analysis. The results showed that positive rate of PRRSV was 9.58% in the collected samples. Phylogenetic analysis of GP5 showed that these strains were clustered into two lineages (1 and 8) indicating different genotypes of PRRSV were circulating in Shandong province. Meanwhile, sequence analysis Of nsp2 showed that the PRRSV strains with 30 amino acids deletions were dominant. Moreover, novel pattern of recombination/deletion and insertion in nsp2 was observed in these strains, indicating that novel PRRSV strains with different patterns of deletions or insertions in nsp2 are emerging in China. All the results suggested that continuous surveillance of PRRSV in China is warranted. Keywords: PRRSV; GP5; nsp2; genetic analysis; Shandong.
猪繁殖与呼吸综合征病毒（PRRSV）是中国养猪业中最重要的经济病原体之一。为了研究 PRRSV 的感染和遗传变异，我们从山东患病猪中采集了 637 份组织样本，并对其进行了 PRRSV 检测。我们对 nsp2 和 ORF5 基因进行了测序，以调查变异和系统发育分析。结果表明，采集的样本中 PRRSV 的阳性率为 9.58%。对 GP5 的系统发育分析表明，这些毒株分为两个谱系（1 和 8），表明山东省存在不同基因型的 PRRSV。同时，nsp2 的序列分析表明，30 个氨基酸缺失的 PRRSV 株占优势。此外，我们在这些毒株中观察到 nsp2 中重组/缺失和插入的新模式，表明中国正在出现具有不同缺失或插入模式的新型 PRRSV 株。所有结果表明，有必要对中国的 PRRSV 进行持续监测。关键词：PRRSV；GP5；nsp2；遗传分析；山东。
