鉴定头颈部鳞状细胞癌预后的增强子 RNA。

Identification of enhancer RNAs for the prognosis of head and neck squamous cell carcinoma.

机构信息

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.

Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.

出版信息

Head Neck. 2021 Dec;43(12):3820-3831. doi: 10.1002/hed.26877. Epub 2021 Sep 27.

DOI:10.1002/hed.26877

PMID:34569097

Abstract

BACKGROUND

Enhancer RNAs (eRNAs) play an important role in carcinogenesis. The landscape of eRNAs in head and neck squamous cell carcinoma (HNSCC) remains largely unknown.

METHODS

The eRNA expression matrix was obtained from the enhancer RNA in the cancer database. Functional enrichment analyses were performed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG). Prognostic eRNAs were identified using Cox regression analysis, and a prognostic prediction model was constructed based on coefficients.

RESULTS

KEGG analysis showed that eRNA-related transcription factors were mainly enriched in herpes simplex virus 1 (HSV1) infection. The zinc finger (ZNF) family may play an essential role in HNSCC. ENSR00000188847, ENSR00000250663, ENSR00000313345, ENSR00000317887, and ENSR00000336429 were identified. The prediction model was robust.

CONCLUSIONS

We constructed a robust 5-eRNA prognostic prediction model, and these eRNAs are potential biomarkers for HNSCC prognosis.

摘要

背景

增强子 RNA（eRNAs）在致癌作用中发挥着重要作用。头颈部鳞状细胞癌（HNSCC）中的 eRNA 图谱在很大程度上尚不清楚。

方法

从癌症数据库中的增强子 RNA 中获得 eRNA 表达矩阵。使用基因本体论和京都基因与基因组百科全书（KEGG）进行功能富集分析。使用 Cox 回归分析鉴定预后 eRNA，并根据系数构建预后预测模型。

结果

KEGG 分析表明，eRNA 相关转录因子主要富集于单纯疱疹病毒 1（HSV1）感染。锌指（ZNF）家族可能在 HNSCC 中发挥重要作用。鉴定到 ENSR00000188847、ENSR00000250663、ENSR00000313345、ENSR00000317887 和 ENSR00000336429。该预测模型具有稳健性。