Department of Zoology, Vidyasagar University, Paschim Medinipur, Midnapore, India.
Department of Biotechnology, Techno India University, Kolkata, India.
Cancer Invest. 2021 Nov;39(10):854-870. doi: 10.1080/07357907.2021.1986060. Epub 2021 Oct 11.
The novel anti-neoplastic glycopeptide T11TS retards glioma both in clinical samples and models. This study investigates the correlation between altering the glioma microenvironment with glioma arrest and death. Flow cytometry, immunoblotting, ELISA, and co-immunoprecipitation were employed to investigate glioma cell arrest and death. Results include a decline in phosphorylation of Akt and attenuation of p21 phosphorylation (Thr145,Ser146) and disassociation of p-Akt-Mdm2 and p-Akt-BAD facilitating death by Akt>BAD. T11TS influence phosphorylation patterns in two focal axes Akt>p21 and Akt>Mdm2>p53. The current article provides crucial insight in deciphering the mechanism of T11TS induced glioma cell arrest and death.
新型抗肿瘤糖肽 T11TS 可延缓临床样本和模型中的神经胶质瘤。本研究探讨了改变神经胶质瘤微环境与神经胶质瘤阻滞和死亡的相关性。采用流式细胞术、免疫印迹、ELISA 和共免疫沉淀法研究神经胶质瘤细胞阻滞和死亡。结果包括 Akt 磷酸化减少，p21 磷酸化（Thr145，Ser146）减弱，p-Akt-Mdm2 和 p-Akt-BAD 解偶联，导致 Akt>BAD 介导的死亡。T11TS 影响 Akt>p21 和 Akt>Mdm2>p53 两个焦点轴上的磷酸化模式。本文为破译 T11TS 诱导的神经胶质瘤细胞阻滞和死亡的机制提供了重要的见解。
