Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
Department of Genetics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
Virulence. 2021 Dec;12(1):2571-2582. doi: 10.1080/21505594.2021.1980989.
infections are the most prevalent cause of serious human mycoses and are the third most common pathogens isolated from bloodstream infections in hospitalized patients. is a member of the spp., which have a predilection for causing life-threatening disease in neonates and hospitalized pediatric patients. In this study, we utilized a infection model to analyze the immunological responses to . Our results demonstrate that the Toll pathway in controls proliferation as the Toll signaling mutant flies are highly susceptible to . We also confirmed that the fly is a convenient invertebrate animal model to analyze virulence properties of different species and strains from the complex as proved to be less virulent than and the -mannan deficient Δ/Δ strain showed attenuated pathogenicity in this immunodeficient background. We also found that Persephone protease is not required for detection and activation of Toll pathway during infection. Furthermore, we observed that β-glucan receptor deficient flies where more sensitive to compared to wild-type flies; however, we could not find a clear dependence on the recognition of this receptor and the cell wall β-glucan exposure-induced host response. These studies establish this infection model as an efficient tool in deciphering immune responses to as well as for assessing virulence factors produced by this emerging fungal predator.
感染是导致人类严重真菌感染的最常见原因，也是住院患者血流感染中分离出的第三大常见病原体。是 spp. 的成员之一，该菌群偏爱导致新生儿和住院儿科患者发生危及生命的疾病。在这项研究中，我们利用感染模型来分析对的免疫反应。我们的结果表明，Toll 通路在中控制增殖，因为 Toll 信号突变体 苍蝇对高度敏感。我们还证实， 苍蝇是一种方便的无脊椎动物模型，可用于分析不同种属和 复合体菌株的毒力特性，与 和缺乏 -mannan 的 Δ/Δ 菌株相比，表现出较低的毒力，在这种免疫缺陷背景下，致病性减弱。我们还发现，在 感染过程中，Persephone 蛋白酶不是检测和激活 Toll 通路所必需的。此外，我们观察到缺乏 β-葡聚糖受体的苍蝇比野生型苍蝇对更敏感；然而，我们不能确定这一受体的识别以及细胞壁 β-葡聚糖暴露诱导的宿主反应是否存在明显的依赖性。这些研究确立了这种 感染模型作为破译对 免疫反应以及评估这种新兴真菌捕食者产生的毒力因子的有效工具。
