1 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Antalya Burdur Yolu, 15030 Yakaköy, Burdur, Turkey.
2 Veterinarian in Gaziantep, Turkey.
Acta Vet Hung. 2021 Sep 24;69(3):291-297. doi: 10.1556/004.2021.00038.
The aim of this study was to determine the effects of gallic acid (GA) on frozen-thawed goat spermatozoa. Four Honamli goat bucks were used at their breeding season, and ejaculates were collected by an electroejaculator. Mixed semen was divided into the following four groups: control (0 mM), low (L; 1 mM), medium (M; 2 mM), and high (H; 4 mM) concentration of GA. All the groups were frozen and thawed in a water bath for spermatological evaluation. The lowest motility was observed in the control group (47.60 ± 5.70%) (P < 0.05), while the highest viability (62.45 ± 1.68%), plasma membrane and acrosome integrity (44.81 ± 4.57%), and high mitochondrial membrane potential (35.96 ± 2.50%) were observed in the low GA group (P < 0.05). Also, the lowest hypo-osmotic swelling test (HOS +) value was found in the high GA group (47.60 ± 4.82%) (P < 0.05). In conclusion, supplementing a low concentration (1 mM) of GA to the Tris-based semen extender had a positive effect on spermatological parameters after freeze-thawing of Honamli goat semen. Further studies should be continued in other species with different doses and combinations using commercial and/or homemade semen extenders.
本研究旨在确定没食子酸（GA）对冷冻解冻山羊精子的影响。在繁殖季节使用 4 只河南黑山羊公山羊，通过电刺激法采集精液。混合精液分为以下四组：对照组（0mM）、低浓度组（L；1mM）、中浓度组（M；2mM）和高浓度组（H；4mM）GA。所有组都在水浴中冷冻和解冻，进行精子学评估。对照组的最低运动性（47.60 ± 5.70%）（P < 0.05），而低 GA 组的最高活力（62.45 ± 1.68%）、质膜和顶体完整性（44.81 ± 4.57%）和高线粒体膜电位（35.96 ± 2.50%）（P < 0.05）。此外，高 GA 组的低渗透压肿胀试验（HOS+）值最低（47.60 ± 4.82%）（P < 0.05）。总之，在 Tris 基精液稀释液中添加低浓度（1mM）GA 对河南黑山羊精液冷冻解冻后的精子学参数有积极影响。应使用商业和/或自制精液稀释液，在其他物种中继续用不同剂量和组合进行进一步研究。
