Roy S, Gold D P, Leskowitz S
J Immunol. 1986 May 1;136(9):3160-5.
Immunization of mice with ABA coupled to carriers to which they are nonresponders gives rise to ABA-specific proliferative responses in lymph node cells. When C3H/HeN and CBA/J nonresponder mice are immunized with ABA on (T,G)-A-L (an I-A-restricted carrier in responder mice), the responses to ABA-tyr and ABA coupled to a variety of unrelated carriers are solely I-A restricted as determined by inhibition with anti-IA and anti-I-E sera. When ABA on GLT (an I-E-restricted carrier in responder mice) is used for immunization, the responses are both I-A and I-E restricted. Thus, ABA-specific responses in nonresponder mice appear in part to be restricted by the carrier used for immunization. B10.S mice, lacking functional I-E molecules, channel their ABA-specific responses entirely through I-A when immunized with ABA-GLT. These results support the hypothesis that the failure in nonresponders lies in a functional deficit in the T cell repertoire rather than an inability of accessory cells to present antigen.
用与无反应小鼠不反应的载体偶联的ABA免疫小鼠，可在淋巴结细胞中引发ABA特异性增殖反应。当C3H/HeN和CBA/J无反应小鼠用（T，G）-A-L（反应小鼠中的一种I-A限制性载体）上的ABA免疫时，如用抗I-A和抗I-E血清抑制所确定的，对ABA- tyr和与多种无关载体偶联的ABA的反应仅受I-A限制。当使用GLT（反应小鼠中的一种I-E限制性载体）上的ABA进行免疫时，反应受I-A和I-E两者限制。因此，无反应小鼠中的ABA特异性反应部分似乎受用于免疫的载体限制。缺乏功能性I-E分子的B1o.S小鼠在用ABA-GLT免疫时，其ABA特异性反应完全通过I-A进行。这些结果支持这样一种假说，即无反应者中的缺陷在于T细胞库的功能缺陷，而不是辅助细胞提呈抗原的无能。
