State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha, 410081, PR China.
State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha, 410081, PR China.
Biosens Bioelectron. 2022 Jan 1;195:113645. doi: 10.1016/j.bios.2021.113645. Epub 2021 Sep 22.
Various sensing platforms based on molecular or nanosystems are widely exploited through molecular diversity and specific recognition. However, it is extremely challenging to develop systems with tunable sensing ability and utilize the systems as information carriers/covers for communication and safety. Herein, DNA nanosensing systems based on cobalt oxyhydroxide (CoOOH) nanosheets were constructed for tunable detection and valence distinction of metal ions, molecular crypto-steganography, and information coding. CoOOH nanosheets absorb fluorescence-labeled single-stranded DNA with different bases and lengths, resulting in fluorescence quenching. The binding priority of bases with CoOOH nanosheets was guanine (G) > cytosine (C) > adenine (A) ≈ thymine (T) and the short chain excelled long chain. Due to the differences in the interaction among CoOOH, DNA, metal ions and variability of DNA bases, various DNA-CoOOH nanosystems have significantly different selective response patterns (that is selectivity) to metal ions and tunable linear ranges to Fe, Hg, Cr. Interestingly, by utilizing their molecular diversity, recognition, selective patterns, DNA-CoOOH sensing systems can be served as doubly cryptographic and steganographic systems to implement information encoding, encryption, and hiding and to reversely improve the selectivity of metal ions. This study provides an idea and platform for adjustable detection and valence distinction of metal ions, and gives a set of "molecular programming languages" for designing intelligent programmable sensing and molecular information communication and safety systems.
各种基于分子或纳米系统的传感平台通过分子多样性和特异性识别得到了广泛的应用。然而，开发具有可调传感能力的系统并利用这些系统作为信息载体/覆盖物进行通信和安全仍然极具挑战性。在此，我们构建了基于钴氢氧化物（CoOOH）纳米片的 DNA 纳米传感系统，用于可调检测和金属离子价态区分、分子密码术和信息编码。CoOOH 纳米片可以吸收具有不同碱基和长度的荧光标记的单链 DNA，从而导致荧光猝灭。碱基与 CoOOH 纳米片的结合优先顺序为鸟嘌呤（G）>胞嘧啶（C）>腺嘌呤（A）≈胸腺嘧啶（T），短链优于长链。由于 CoOOH、DNA、金属离子之间的相互作用以及 DNA 碱基的可变性的差异，各种 DNA-CoOOH 纳米系统对金属离子具有明显不同的选择性响应模式（即选择性），并且对 Fe、Hg、Cr 具有可调的线性范围。有趣的是，通过利用它们的分子多样性、识别、选择性模式，DNA-CoOOH 传感系统可以作为双重加密和隐写系统，实现信息编码、加密和隐藏，并反向提高金属离子的选择性。本研究为金属离子的可调检测和价态区分提供了一种思路和平台，并为设计智能可编程传感和分子信息通信与安全系统提供了一套“分子编程语言”。
