Laboratory of Virology, Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland.
Department of Infectious Diseases and Hepatology, Medical University of Lodz, 91-347 Lodz, Poland.
Cells. 2021 Sep 8;10(9):2360. doi: 10.3390/cells10092360.
Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and are essential components of the host's innate immune response. The aim of this study was to determine the genotype frequency and investigate the association between polymorphisms and cytomegalovirus (CMV) DNAemia in human immunodeficiency virus (HIV)/CMV co-infected patients. A total of 205 HIV/CMV co-infected adults were screened for the presence of the four polymorphisms (-1237T/C, -1486T/C, 1174G/A, and 2848C/T) by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Mutation presented in at least one allele of the 2848C/T single nucleotide polymorphism (SNP) was associated with the occurrence of CMV DNAemia among HIV-infected patients with CMV co-infection ( = 0.004). The level of CMV DNA was higher in patients who were homozygous recessive or heterozygous for the 2848C/T polymorphism compared with those who had a wild-type genotype for this polymorphism ( = 0.005). Mutation detected in at least one allele of this SNP was also associated with a lower interferon type β (IFN-β) concentration ( = 0.048), while no relationships between -1237T/C, -1486T/C, and 1174G/A SNPs and CMV DNAemia were observed. Our findings suggest that the mutation present in at least one allele of the 2848C/T SNP may be associated with the active CMV infection in HIV/CMV co-infected subjects.
Toll 样受体 (TLRs) 识别病原体相关分子模式，是宿主固有免疫反应的重要组成部分。本研究旨在确定基因型频率，并探讨人类免疫缺陷病毒 (HIV)/巨细胞病毒 (CMV) 合并感染患者中 TLR 基因多态性与 CMV 血症的关系。采用聚合酶链反应-限制性片段长度多态性 (PCR-RFLP) 法对 205 例 HIV/CMV 合并感染的成年人进行了 4 个 TLR 基因多态性 (-1237T/C、-1486T/C、1174G/A 和 2848C/T) 的检测。结果显示，至少一个等位基因存在 2848C/T 单核苷酸多态性 (SNP) 突变与 HIV 合并 CMV 感染患者发生 CMV 血症有关 (=0.004)。与野生型基因型相比，2848C/T 多态性纯合隐性或杂合突变的患者 CMV DNA 水平更高(=0.005)。该 SNP 至少一个等位基因的突变与 IFN-β浓度降低相关(=0.048)，而 -1237T/C、-1486T/C 和 1174G/A 三个 SNP 与 CMV 血症之间无相关性。综上所述，至少一个等位基因存在 2848C/T SNP 突变可能与 HIV/CMV 合并感染患者的 CMV 活动性感染有关。
