RNA Bioscience Initiative, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Cells. 2021 Sep 9;10(9):2376. doi: 10.3390/cells10092376.
Adrenal steroid hormone production is a dynamic process stimulated by adrenocorticotropic hormone (ACTH) and angiotensin II (AngII). These ligands initialize a rapid and robust gene expression response required for steroidogenesis. Here, we compare the predominant human immortalized cell line model, H295R cell, with primary cultures of adult adrenocortical cells derived from human kidney donors. We performed temporally resolved RNA-seq on primary cells stimulated with either ACTH or AngII at multiple time points. The magnitude of the expression dynamics elicited by ACTH was greater than AngII in primary cells. This is likely due to the larger population of adrenocortical cells that are responsive to ACTH. The dynamics of stimulus-induced expression in H295R cells are mostly recapitulated in primary cells. However, there are some expression responses in primary cells absent in H295R cells. These data are a resource for the endocrine community and will help researchers determine whether H295R is an appropriate model for the specific aspect of steroidogenesis that they are studying.
肾上腺类固醇激素的产生是一个受促肾上腺皮质激素 (ACTH) 和血管紧张素 II (AngII) 刺激的动态过程。这些配体启动了类固醇生成所需的快速而强大的基因表达反应。在这里，我们将比较主要的人类永生化细胞系模型 H295R 细胞与源自人类肾脏供体的成人肾上腺皮质细胞的原代培养物。我们对原代细胞进行了时间分辨的 RNA-seq 分析，这些细胞分别用 ACTH 或 AngII 刺激，并在多个时间点进行了分析。在原代细胞中，ACTH 诱导的表达动力学幅度大于 AngII。这可能是由于对 ACTH 有反应的肾上腺皮质细胞数量较多。H295R 细胞中刺激诱导表达的动力学在原代细胞中大多得到了重现。然而，在原代细胞中存在一些在 H295R 细胞中不存在的表达反应。这些数据是内分泌学界的一个资源，将帮助研究人员确定 H295R 是否是他们正在研究的类固醇生成特定方面的合适模型。
