Söderström K O, Parvinen M
Acta Anat (Basel). 1978;100(4):557-72. doi: 10.1159/000144940.
In the seminiferous tubules of the rat, as in most mammalian species, the developing germ cells form associations with constant cell composition. These cellular associations or stages follow each other in a regular manner along the seminiferous tubules giving rise to seminiferous epithelial wave. When a freshly isolated unstained seminiferous tubulus of the rat is subjected to transillumination under a stereomicroscope, the different segments of the seminiferous epithelial wave absorb light in a characteristic manner permitting their recognition. Using this technique, small segments with accurately known cell composition can be isolated and studied in living state with phase-contrast microscopy. In several cases, the phase-contrast microscopy gives more information about the cell morphology than conventional histological methods. In this study all major developmental steps from early spermatogonia to mature spermatids have been described. The findings of the present study can be used as reference material in the evaluation and identification of the various cell types of the seminiferous tubules obtained, e.g. by the Staput fractionation method. In addition, the findings may be helpful in the evaluation of spermatogenic and Sertoli cells in culture conditions.
与大多数哺乳动物一样，在大鼠的生精小管中，发育中的生殖细胞会形成细胞组成恒定的细胞组合。这些细胞组合或阶段沿着生精小管以规则的方式相继出现，形成生精上皮波。当将新鲜分离的未染色大鼠生精小管在体视显微镜下进行透照时，生精上皮波的不同节段会以特征性方式吸收光线，从而得以识别。利用这项技术，可以分离出细胞组成精确已知的小片段，并通过相差显微镜在活体状态下进行研究。在一些情况下，相差显微镜比传统组织学方法能提供更多关于细胞形态的信息。在本研究中，描述了从早期精原细胞到成熟精子细胞的所有主要发育步骤。本研究的结果可作为参考材料，用于评估和鉴定例如通过斯塔普特分级分离法获得的生精小管的各种细胞类型。此外，这些结果可能有助于评估培养条件下的生精细胞和支持细胞。
