Hossein Mohammad Shamim, Yu Xianfeng, Son Young-Bum, Jeong Yeon-Ik, Jeong Yeon-Woo, Choi Eun-Ji, Tinson Alex H, Singh Kuhad Kuldip, Singh Rajesh, Noura Al Shamsi, Hwang Woo-Suk
UAE Biotech Research Center, Al Wathba South, Abu Dhabi 30310, United Arab Emirates.
Jilin Provincial Key Laboratory of Animal Model, College of Animal Science, Jilin University, Changchun 130062, China.
Animals (Basel). 2021 Sep 14;11(9):2691. doi: 10.3390/ani11092691.
Somatic cell nuclear transfer (SCNT) provides a unique opportunity to reproduce animals with superior genetics. Viable cell lines are usually established from tissues collected by biopsy from living animals in the SCNT program. In the present study, tissues were collected and preserved from a suddenly deceased champion camel. We established cell lines from these decade-old tissues and used them as nuclear donors. After 42 h of in vitro maturation, 68.00 ± 2.40% of oocytes reached the metaphase II (M II) stage while 87.31 ± 2.57% in vivo collected oocytes were matured at collection ( < 0.05). We observed a higher blastocyst formation rate when in vivo matured oocytes (43.45 ± 2.07%) were used compared to in vitro matured oocytes (21.52 ± 1.74%). The live birth rate was 6.45% vs. 16.67% for in vitro and in vivo matured oocytes, respectively. Microsatellite analysis of 13 camel loci revealed that all the SCNT-derived offspring were identical to each other and with their somatic cell donor. The present study succeeded in the resurrection of 11 healthy offspring from the decade-old vitrified tissues of a single somatic cell donor individual using both in vitro and in vivo matured oocytes.
体细胞核移植（SCNT）为繁殖具有优良基因的动物提供了独特的机会。在体细胞核移植项目中，可行的细胞系通常从活体动物活检采集的组织中建立。在本研究中，从一头突然死亡的冠军骆驼身上采集并保存了组织。我们从这些保存了十年的组织中建立了细胞系，并将其用作核供体。体外成熟42小时后，68.00±2.40%的卵母细胞达到减数第二次分裂中期（M II期），而体内采集的卵母细胞在采集时的成熟率为87.31±2.57%（P<0.05）。我们观察到，与体外成熟的卵母细胞（21.52±1.74%）相比，使用体内成熟的卵母细胞时囊胚形成率更高（43.45±2.07%）。体外和体内成熟卵母细胞的活产率分别为6.45%和16.67%。对13个骆驼基因座的微卫星分析表明，所有体细胞核移植衍生后代彼此相同，且与它们的体细胞供体相同。本研究成功地利用体外和体内成熟的卵母细胞，从单个体细胞供体个体保存了十年的玻璃化组织中复活了11个健康后代。
