Burca-Busaga Cristina Gabriela, Betoret Noelia, Seguí Lucía, García-Hernández Jorge, Hernández Manuel, Barrera Cristina
Instituto Universitario de Ingeniería de Alimentos para el Desarrollo de la Universitat Politècnica de València, 46022 Valencia, Spain.
Centro Avanzado de Microbiología de Alimentos de la Universitat Politècnica de València, 46022 Valencia, Spain.
Foods. 2021 Sep 12;10(9):2155. doi: 10.3390/foods10092155.
To benefit the health of consumers, bioactive compounds must reach an adequate concentration at the end of the digestive process. This involves both an effective release from the food matrix where they are contained and a high resistance to exposure to gastrointestinal conditions. Accordingly, this study evaluates the impact of trehalose addition (10% ) and homogenization (100 MPa), together with the structural changes induced in vacuum impregnated apple slices (VI) by air-drying (AD) and freeze-drying (FD), on spp. (CECT 4063) survival and the bioaccessibility of antioxidants during in vitro digestion. Vacuum impregnated apple slices conferred maximum protection to the lactobacillus strain during its passage through the gastrointestinal tract, whereas drying with air reduced the final content of the living cells to values below 10 cfu/g. The bioaccessibility of antioxidants also reached the highest values in the VI samples, in which the release of both the total phenols and total flavonoids to the liquid phase increased with in vitro digestion. The addition of trehalose and homogenization at 100 MPa increased the total bioaccessibility of antioxidants in FD and AD apples and the total bioaccessibility of flavonoids in the VI samples. Homogenizing at 100 MPa also increased the survival of during in vitro digestion in FD samples.
为使消费者健康受益，生物活性化合物必须在消化过程结束时达到足够的浓度。这既涉及它们从所含食物基质中的有效释放，也涉及对胃肠道环境暴露的高耐受性。因此，本研究评估了添加海藻糖（10%）和均质化（100兆帕），以及通过风干（AD）和冷冻干燥（FD）对真空浸渍苹果片（VI）诱导的结构变化，对 spp. （CECT 4063）存活以及体外消化过程中抗氧化剂生物可及性的影响。真空浸渍苹果片在乳酸菌菌株通过胃肠道的过程中提供了最大程度的保护，而风干则使活细胞的最终含量降至低于10 cfu/g的值。抗氧化剂的生物可及性在VI样品中也达到了最高值，其中总酚和总黄酮向液相中的释放随着体外消化而增加。添加海藻糖和100兆帕的均质化提高了FD和AD苹果中抗氧化剂的总生物可及性以及VI样品中黄酮类化合物的总生物可及性。在100兆帕下进行均质化还提高了FD样品在体外消化过程中 的存活率。
