Suzuki S
Bull Tokyo Med Dent Univ. 1986 Mar;33(1):23-34.
The purpose of this study was to carry out the histomorphometric assay on the mandibular condylar tissue of the growing rats. Male rats of the Wistar strain at the age of 3, 4, 6, 8 and 10 weeks were used. All rats were injected intravenously with tetracycline and calcein, respectively. They were killed 12 hours after calcein injection. Before embedding in acrylic resin, 3 reference points were marked directly on each condyle to establish the reference plane for the histomorphometry on the ground section. After preparing the ground section, the growth rate of the condyle was obtained by measuring the distance of the two different fluorescent labeling lines. Furthermore, the age-related changes of the relative distribution of the bone, calcified cartilage and prebone (uncalcified bone matrix) were measured under a light microscope by using a grid on the eyepiece reticle. The number of osteoblasts and osteoclasts were also calculated in the same specimen. The following results were obtained: The thickness of the cartilaginous layer and the growth rate of the endochondral bone formation decreased with age. The relative ratio of the bone area in the subchondral tissue increased constantly with age.
本研究的目的是对生长中大鼠的下颌髁突组织进行组织形态计量学分析。使用3、4、6、8和10周龄的Wistar品系雄性大鼠。所有大鼠分别静脉注射四环素和钙黄绿素。在注射钙黄绿素12小时后将它们处死。在嵌入丙烯酸树脂之前，直接在每个髁突上标记3个参考点，以建立磨片组织形态计量学的参考平面。制备磨片后，通过测量两条不同荧光标记线之间的距离来获得髁突的生长速率。此外，使用目镜分划板上的网格在光学显微镜下测量骨、钙化软骨和前骨（未钙化骨基质）相对分布的年龄相关变化。在同一标本中还计算了成骨细胞和破骨细胞的数量。获得了以下结果：软骨层厚度和软骨内骨形成的生长速率随年龄降低。软骨下组织中骨面积的相对比例随年龄不断增加。
