Schroedter Linda, Streffer Friedrich, Streffer Katrin, Unger Peter, Venus Joachim
Bioengineering Department, Leibniz Institute for Agricultural Engineering and Bioeconomy e. V. (ATB), 14469 Potsdam, Germany.
LXP Group GmbH, 14513 Teltow, Germany.
Microorganisms. 2021 Aug 25;9(9):1810. doi: 10.3390/microorganisms9091810.
A new biorefinery concept is proposed that integrates the novel LX-Pretreatment with the fermentative production of L-(+)-lactic acid. Lignocellulose was chosen as a substrate that does not compete with the provision of food or feed. Furthermore, it contains lignin, a promising new chemical building material which is the largest renewable source for aromatic compounds. Two substrates were investigated: rye straw (RS) as a residue from agriculture, as well as the fibrous digestate of an anaerobic biogas plant operated with energy corn (DCS). Besides the prior production of biogas from energy corn, chemically exploitable LX-Lignin was produced from both sources, creating a product with a low carbohydrate and ash content (90.3% and 88.2% of acid insoluble lignin). Regarding the cellulose fraction of the biomass, enzymatic hydrolysis and fermentation experiments were conducted, comparing a separate (SHF), simultaneous (SSF) and prehydrolyzed simultaneous saccharification and fermentation (PSSF) approach. For this purpose, thermophilic 14-300 was utilized, reaching 38.0 g L LA in 32 h SSF from pretreated RS and 18.3 g L LA in 30 h PSSF from pretreated DCS with optical purities of 99%.
提出了一种新的生物炼制概念，即将新型LX预处理与L-(+)-乳酸的发酵生产相结合。选择木质纤维素作为不与食品或饲料供应竞争的底物。此外，它含有木质素，这是一种很有前景的新型化学建筑材料，也是芳香族化合物的最大可再生来源。研究了两种底物：作为农业残留物的黑麦草（RS）以及以能源玉米为原料运行的厌氧沼气厂的纤维消化物（DCS）。除了先从能源玉米生产沼气外，还从这两种来源生产了可化学利用的LX-木质素，得到了一种碳水化合物和灰分含量低的产品（酸不溶性木质素分别为90.3%和88.2%）。关于生物质的纤维素部分，进行了酶水解和发酵实验，比较了单独水解发酵（SHF）、同步糖化发酵（SSF）和预水解同步糖化发酵（PSSF）方法。为此，使用了嗜热菌14-300，在来自预处理RS的32小时SSF中达到38.0 g/L的L-乳酸，在来自预处理DCS的30小时PSSF中达到18.3 g/L的L-乳酸，光学纯度为99%。
