Gambari R, Del Senno L, Barbieri R, Buzzoni D, Gustafsson K, Giacomini P, Natali P G
Eur J Immunol. 1986 Apr;16(4):365-9. doi: 10.1002/eji.1830160409.
DNA methylation at the 5'-CCGG-3' sites of the HLA-DR alpha gene and relative flanking regions has been analyzed by Msp I and Hpa II enzymatic digestion in order to determine whether a correlation exists between DNA methylation and transcription of the HLA-DR alpha gene. Unexpectedly, and in contrast to the behavior of most eukaryotic genes, no positive correlation was found between hypomethylation and expression. In fact, HLA-DR alpha appears to be fully unmethylated at Msp I/Hpa II sites in K562 cells, not expressing DR molecules, and to exhibit a high degree of methylation in Colo 38 cells, which actively transcribe the gene. The unorthodox behavior of HLA-DR alpha is not unique to melanoma or erythroid cells since a similar, positive correlation between methylation and expression also exists when this analysis is extended to cell lines belonging to other histotypes.
通过Msp I和Hpa II酶切分析了HLA - DRα基因5'-CCGG-3'位点及其相对侧翼区域的DNA甲基化情况，以确定DNA甲基化与HLA - DRα基因转录之间是否存在相关性。出乎意料的是，与大多数真核基因的情况相反，未发现低甲基化与表达之间存在正相关。事实上，在不表达DR分子的K562细胞中，HLA - DRα在Msp I/Hpa II位点似乎完全未甲基化，而在活跃转录该基因的Colo 38细胞中则表现出高度甲基化。HLA - DRα的这种非传统行为并非黑色素瘤或红细胞系细胞所特有，因为当将此分析扩展到其他组织类型的细胞系时，甲基化与表达之间也存在类似的正相关。
