The bioprocessing of chitinous fishery wastes (CFWs) to chitinases through fermentation approaches has gained importance owing to its great benefits in reducing the enzyme production cost, and utilizing chitin waste. In this work, our study of the chitinase production of sp. TKU052 in the presence of different kinds of CFWs revealed a preference for demineralized crab shells powder (deCSP); furthermore, a 72 kDa chitinase was isolated from the 0.5% deCSP-containing medium. The sp. TKU052 chitinase displayed maximum activity at 70 °C and pH 4-5, while Zn, Fe, Triton X-100, Tween 40, and SDS exerted a negative effect on its activity, whereas Mn and 2-mercaptoethanol were found to potentially enhance the activity. Among various kinds of polysaccharide, sp. TKU052 chitinase exhibited the best catalytic activity on colloidal chitin (CC) with K = 9.75 mg/mL and V = 2.43 μmol/min. The assessment of the hydrolysis of CC and -acetyl chitooligosaccharides revealed that sp. TKU052 chitinase possesses multiple catalytic functions, including exochitinase, endochitinase, and -acetyl-β-D-glucosaminidase activities. Finally, the combination of sp. TKU052 chitinase and TKU048 -acetyl-β-D-glucosaminidase could efficiently convert CC to -acetyl-D-glucosamine (GlcNAc) with a production yield of 94.35-98.60% in 12-24 h.