Savić Luka, Augustyniak Edyta M, Kastensson Adele, Snelling Sarah, Abhari Roxanna E, Baldwin Mathew, Price Andrew, Jackson William, Carr Andrew, Mouthuy Pierre-Alexis
Botnar Institute of Musculoskeletal Sciences, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.
Botnar Institute of Musculoskeletal Sciences, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK; Nuffield Orthopaedic Centre, Oxford University Hospitals NHS Trust, Oxford, UK.
Mater Sci Eng C Mater Biol Appl. 2021 Oct;129:112414. doi: 10.1016/j.msec.2021.112414. Epub 2021 Sep 3.
Despite the clinical success of Anterior Cruciate Ligament reconstruction (ACLR) in some patients, unsatisfactory clinical outcomes secondary to graft failure are seen, indicating the need to develop new regeneration strategies. The use of degradable and bioactive textiles has the potential to improve the biological repair of soft tissue. Electrospun (ES) filaments are particularly promising as they have the ability to mimic the structure of natural tissues and influence endogenous cell behaviour. In this study, we produced continuous polycaprolactone (PCL) ES filaments using a previously described electrospinning collection method. These filaments were stretched, twisted, and assembled into woven structures. The morphological, tensile, and biological properties of the woven fabric were then assessed. Scanning electron microscopy (SEM) images highlighted the aligned and ACL-like microfibre structure found in the stretched filaments. The tensile properties indicated that the ES fabric reached suitable strengths for a use as an ACLR augmentation device. Human ACL-derived cell cultured on the fabric showed approximately a 3-fold increase in cell number over 2 weeks and this was equivalent to a collagen coated synthetic suture commonly used in ACLR. Cells generally adopted a more elongated cell morphology on the ES fabric compared to the control suture, aligning themselves in the direction of the microfibres. A NRU assay confirmed that the ES fabric was non-cytotoxic according to regulatory standards. Overall, this study supports the development of ES textiles as augmentation devices for ACLR.
尽管前交叉韧带重建术（ACLR）在部分患者中取得了临床成功，但仍有因移植物失败导致的不理想临床结果出现，这表明需要开发新的再生策略。使用可降解且具有生物活性的纺织品有改善软组织生物修复的潜力。电纺（ES）长丝尤其具有前景，因为它们能够模仿天然组织的结构并影响内源性细胞行为。在本研究中，我们采用先前描述的电纺收集方法制备了连续的聚己内酯（PCL）ES长丝。这些长丝经过拉伸、捻合并组装成机织结构。然后评估了该机织物的形态、拉伸和生物学性能。扫描电子显微镜（SEM）图像突出显示了在拉伸长丝中发现的排列成行且类似前交叉韧带的微纤维结构。拉伸性能表明，该ES织物达到了用作ACLR增强装置的合适强度。在该织物上培养的源自人前交叉韧带的细胞在2周内细胞数量增加了约3倍，这与ACLR中常用的胶原涂层合成缝线相当。与对照缝线相比，细胞在ES织物上通常呈现出更细长的细胞形态，并沿微纤维方向排列。一项中性红摄取（NRU）试验证实，根据监管标准，该ES织物无细胞毒性。总体而言，本研究支持将ES纺织品开发为ACLR的增强装置。
