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锌(II)尼氟灭酸配合物对人子宫内膜细胞系中 MMP 活性和基因表达的影响。

Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines.

机构信息

Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, 040 11, Košice, Slovakia.

Department of Gynaecology and Obstetrics, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia.

出版信息

Sci Rep. 2021 Sep 27;11(1):19086. doi: 10.1038/s41598-021-98512-9.

DOI:10.1038/s41598-021-98512-9
PMID:34580366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8476601/
Abstract

Endometriosis is a chronic inflammatory disease which increasingly affects young women under 35 years of age and leads to subfertility even infertility. Analysis of the cytotoxic effect of zinc(II) niflumato complex with neocuproine ([Zn(neo)(nif)] or Zn-Nif) on immortalized human endometriotic cell line (12Z) and on control immortalized human endometrial stromal cell line (hTERT) was performed using xCELLigence technology for approximately 72 h following the treatment with Zn-Nif as well as cell viability Trypan Blue Assay. 12Z cell line proliferated more slowly compared to unaffected cells, whereas hTERT cells did not show similar behavior after treatment. The complex probably reduces the effect of pro-inflammatory pathways due to the effect of NSAID, while presence of zinc might reduce the level of ROS and regulate ER2 levels and MMP activity. The observed effects and high selectivity for rapidly proliferating cells with increased inflammatory activity suggest a good prognosis of successful decrease of endometriosis stage with this complex.

摘要

子宫内膜异位症是一种慢性炎症性疾病，越来越多地影响 35 岁以下的年轻女性，导致生育能力下降甚至不孕。使用 xCELLigence 技术分析锌（II）硝呋拉太配合新铜试剂络合物（[Zn(neo)(nif)]或 Zn-Nif）对永生化人子宫内膜异位细胞系（12Z）和对照永生化人子宫内膜基质细胞系（hTERT）的细胞毒性作用，在 Zn-Nif 处理后大约 72 小时进行，并通过台盼蓝法测定细胞活力。与未受影响的细胞相比，12Z 细胞系的增殖速度较慢，而 hTERT 细胞在处理后没有表现出类似的行为。该复合物可能由于 NSAID 的作用而降低促炎途径的作用，而锌的存在可能降低 ROS 水平并调节 ER2 水平和 MMP 活性。观察到的对具有增加炎症活性的快速增殖细胞的高选择性表明，该复合物成功降低子宫内膜异位症阶段具有良好的预后。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/6de2ebf4d971/41598_2021_98512_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/a9019613753c/41598_2021_98512_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/b5871b956e57/41598_2021_98512_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/1cd8848f2584/41598_2021_98512_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/364e509f33e2/41598_2021_98512_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/85111e16a0b2/41598_2021_98512_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/6de2ebf4d971/41598_2021_98512_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/a9019613753c/41598_2021_98512_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/f05ddb83fb93/41598_2021_98512_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/c56737c42269/41598_2021_98512_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/b5871b956e57/41598_2021_98512_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/1cd8848f2584/41598_2021_98512_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/364e509f33e2/41598_2021_98512_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/85111e16a0b2/41598_2021_98512_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ce8/8476601/6de2ebf4d971/41598_2021_98512_Fig8_HTML.jpg

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