Angrisani N, Willumeit-Römer R, Windhagen H, Mavila Chathoth B, Scheper V, Wiese B, Helmholz H, Reifenrath J
Medizinische Hochschule Hannover, Stadtfelddamm 34, 30625 Hannover,
Eur Cell Mater. 2021 Sep 28;42:179-195. doi: 10.22203/eCM.v042a14.
No optimal therapy exists to stop or cure chondral degeneration in osteoarthritis (OA). While the pathogenesis is unclear, there is consensus on the etiological involvement of both articular cartilage and subchondral bone. Compared to original bone, the substance of sclerotic bone is mechanically less solid. The osteoproliferative effect of Mg has been shown repeatedly during development of Mg-based osteosynthesis implants. The aim of the present study was to examine the influence of implanted high-purity Mg cylinders on subchondral bone quality in a rabbit OA model. 10 New Zealand White rabbits received into the knee either 20 empty drill holes or 20 drill holes, which were additionally filled with one Mg cylinder each. Follow-up was at 8 weeks. Micro-computed tomography (µCT) was performed. After euthanasia, cartilage condition was determined, bone samples were collected and processed for histological evaluation and elemental imaging by micro-X-ray fluorescence spectrometry (µXRF). Articular cartilage collected post-mortem showed different stages of lesions, from mild alterations up to exposed subchondral bone, which tended to be slightly lower in animals with implanted Mg cylinders. µCT showed significantly increased bone volume in the Mg group. Also, histological evaluation revealed distinct differences. While right, operated limbs did not show any significant difference, left, non-operated controls showed significantly less changes in articular cartilage in the Mg group. A distinct influence of implanted cylinders of pure Mg on subchondral bone of osteoarthritic rabbits was shown. Subsequent evaluations, including other time points and alternative alloys, will show if this could alter OA progression.
目前尚无最佳疗法来阻止或治愈骨关节炎（OA）中的软骨退变。虽然发病机制尚不清楚，但关节软骨和软骨下骨在病因学上的参与已达成共识。与原始骨相比，硬化骨的物质在机械性能上不太坚固。镁在镁基骨合成植入物的开发过程中反复显示出成骨增殖作用。本研究的目的是在兔OA模型中研究植入高纯度镁圆柱体对软骨下骨质量的影响。10只新西兰白兔的膝关节分别接受20个空钻孔或20个钻孔，每个钻孔额外填充一个镁圆柱体。随访时间为8周。进行了微型计算机断层扫描（µCT）。安乐死后，确定软骨状况，收集骨样本并进行处理，以通过微X射线荧光光谱法（µXRF）进行组织学评估和元素成像。死后收集的关节软骨显示出不同阶段的病变, 从轻度改变到软骨下骨暴露，植入镁圆柱体的动物的病变程度往往略低。µCT显示镁组的骨体积显著增加。此外，组织学评估也显示出明显差异。虽然右侧手术肢体没有显示出任何显著差异，但左侧未手术的对照组显示镁组关节软骨的变化明显较少。结果表明，植入的纯镁圆柱体对骨关节炎兔的软骨下骨有明显影响。后续评估，包括其他时间点和替代合金，将表明这是否会改变OA的进展。
