Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Tokai National Higher Education and Research System, Nagoya, Japan.
Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Tokai National Higher Education and Research System, Nagoya, Japan.
Fish Shellfish Immunol. 2021 Nov;118:405-410. doi: 10.1016/j.fsi.2021.09.029. Epub 2021 Sep 25.
The mechanism via which the mothers of viviparous animals regulate the internal environment of pregnancy-associated organs for maintaining offspring growth is poorly understood. Environmental niches in organs contain fluid components for supporting embryonic growth; however, they may serve as nutrients for microbes. Therefore, microbial control is essential in viviparous animals to reduce the risk of infection in the ovarian lumen. Its importance may be higher than that in the case of oviparous animals. In this study, we investigated the antimicrobial factors in a viviparous teleost, Xenotoca eiseni. Four transcripts of the liver-expressed antimicrobial peptide (LEAP) were identified via RNA-Seq analysis. Some of the genes were expressed in the ovaries or intraovarian embryos of the fish. In particular, high expression of leap1a was detected in the ovaries of both pregnant and non-pregnant fish. Moreover, the ovary extracts from X. eiseni and transformed leap genes exhibited antimicrobial activity against Escherichia coli. Our results suggest that viviparous teleosts utilize antimicrobial peptides to reduce the risk of infection in the ovarian lumen.
尚不清楚胎生动物的母体通过何种机制来调节与妊娠相关的器官内环境，以维持后代的生长。器官中的环境小生境包含支持胚胎生长的液体成分；然而，它们也可能是微生物的营养来源。因此，在胎生动物中，微生物的控制对于降低卵巢腔感染的风险至关重要，其重要性可能高于卵生动物。在这项研究中，我们调查了胎生硬骨鱼 Xenotoca eiseni 的抗微生物因子。通过 RNA-Seq 分析鉴定了四个肝脏表达的抗菌肽 (LEAP) 的转录本。这些基因中的一些在鱼的卵巢或卵巢内胚胎中表达。特别是，在怀孕和未怀孕的鱼的卵巢中检测到 leap1a 的高表达。此外，X. eiseni 的卵巢提取物和转化的 leap 基因表现出对大肠杆菌的抗菌活性。我们的结果表明，胎生硬骨鱼利用抗菌肽来降低卵巢腔感染的风险。
