National Forensic Service, Wonju, Gangwon-do, Korea.
J Forensic Sci. 2022 Jan;67(1):353-357. doi: 10.1111/1556-4029.14896. Epub 2021 Sep 29.
Cyanide is a highly toxic agent that has been frequently used for suicide in South Korea. It is also used in various industrial fields, such as metal plating, in which many accidental cyanide intoxications have occurred. To overcome the disadvantages of conventional cyanide analysis methods, a simple and fast method for the analysis of cyanide in whole blood using ion chromatography (IC) with amperometric detection was developed in this study. Whole blood samples were deproteinized, diluted, and analyzed using an IC-amperometric detection system. The limits of detection and quantitation were 0.1 and 0.2 mg/L, respectively. The method showed good linearity in the range of 0.2 to 50 mg/L with R > 0.99. The intra- and inter-assay precision and accuracy values were <10%. The established method was successfully applied to analyze whole blood samples from three cyanide intoxication cases.
氰化物是一种剧毒物质，在韩国被频繁用于自杀。它也被广泛应用于各种工业领域，如金属电镀，其中发生了许多意外的氰化物中毒事件。为了克服传统氰化物分析方法的缺点，本研究开发了一种使用离子色谱（IC）与安培检测法分析全血中氰化物的简单快速方法。本研究采用蛋白沉淀、稀释和 IC-安培检测系统分析全血样本。检测限和定量限分别为 0.1 和 0.2 mg/L。该方法在线性范围为 0.2 至 50 mg/L 内具有良好的线性关系，R ＞0.99。日内和日间精密度和准确度值均＜10%。所建立的方法成功地应用于分析 3 例氰化物中毒全血样本。
