Perkins S L, Yunis A A
Exp Hematol. 1986 Jun;14(5):401-5.
HL-60 cells secrete an autostimulatory activity (ASA) that appears to be required for their growth in vitro. The purpose of this study was to examine the pattern of colony-stimulating activity in conditioned media of HL-60 after induction of differentiation by the phorbol ester phorbol-12-myristate-13-acetate (PMA). PMA-induced differentiation to monocyte-macrophage cells was accompanied by persistence of ASA secretion and the appearance of new species of colony-stimulating activities similar in properties to human CSF I and CSF II. Thus the secretion of ASA by HL-60 appears to be a characteristic property of these cells that is maintained after terminal differentiation.
HL-60细胞分泌一种自身刺激活性（ASA），这似乎是它们在体外生长所必需的。本研究的目的是检测经佛波酯佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）诱导分化后HL-60条件培养基中的集落刺激活性模式。PMA诱导分化为单核细胞-巨噬细胞时，伴随着ASA分泌的持续存在以及出现了性质类似于人CSF I和CSF II的新集落刺激活性种类。因此，HL-60分泌ASA似乎是这些细胞的一个特征性特性，在终末分化后仍保持。
