Cossu G, Kuo A L, Pessano S, Warren L, Cooper R A
Cancer Res. 1982 Feb;42(2):484-9.
The human promyelocytic leukemia ell line, HL-60, synthesized a class of high-molecular-weight (M.W. 5000 to 7000), N-linked glycopeptides as the major class of protein-bound carbohydrates. Small glycopeptides (M.W. 2500 to 3500), typical of most mammalian cells except erythrocytes, represented a minor component in these cells. The large glycopeptides were labeled efficiently with fucose, glucosamine, and galactose but only poorly with mannose. They were found not to be glycolipids, glycosaminoglycans, or mucin-type glycopeptides and were not susceptible to exoglycosidases, but they were partially degraded by endo-beta-galactosidases. These characteristics are similar to those of the large glycopeptides synthesized by erythrocytes, by another human myeloid leukemia cell line (K562), and by human and murine teratocarcinoma cells. High-molecular-weight glycopeptides predominated on another human myeloid leukemia cell line KG1, but they were expressed at low levels on both a human monocytic leukemia cel line (THP-1) and a human T-lymphoblastoid cell line (Jurkat). When HL-60 cells were induced to differentiate into macrophage-like cells with phorbol esters, the proportion of large glycopeptides decreased, and the production of small glycopeptides predominated. This shift was observed within the first several hr after exposure to phorbol esters and was temporally related to the acquisition of adherent properties by the induced cells. In contrast, when HL-60 cells were induced to differentiate into granulocytes by dimethyl sulfoxide, hypoxanthine, or retinoic acid, they continued to synthesize glycopeptides similar to uninduced cells. Human peripheral blood granulocytes synthesized primarily large glycopeptides, whereas monocytes and lymphocytes synthesized mostly small glycopeptides. These results indicate that the synthesis of high-molecular-weight glycopeptides is a property of human myeloid leukemia cell lines and that it persists throughout myeloid differentiation. A proportionate decrease in the synthesis of these large glycopeptidase is a part of the differentiation program for monocytes and macrophages.
人早幼粒细胞白血病细胞系HL - 60合成了一类高分子量（分子量5000至7000）的N - 连接糖肽，作为蛋白质结合碳水化合物的主要类别。小糖肽（分子量2500至3500），除红细胞外大多数哺乳动物细胞所具有的典型特征，在这些细胞中占次要成分。大糖肽被岩藻糖、葡糖胺和半乳糖有效标记，但被甘露糖标记的效率很低。发现它们不是糖脂、糖胺聚糖或粘蛋白型糖肽，且不易被外切糖苷酶作用，但可被内切β - 半乳糖苷酶部分降解。这些特征与红细胞、另一种人髓系白血病细胞系（K562）以及人和鼠的畸胎癌细胞合成的大糖肽相似。高分子量糖肽在另一种人髓系白血病细胞系KG1上占主导地位，但在人单核细胞白血病细胞系（THP - 1）和人T淋巴细胞样细胞系（Jurkat）上表达水平较低。当用佛波酯诱导HL - 60细胞分化为巨噬细胞样细胞时，大糖肽的比例下降，小糖肽的产生占主导。这种转变在接触佛波酯后的最初几个小时内就可观察到，并且在时间上与诱导细胞获得贴壁特性相关。相反，当用二甲亚砜、次黄嘌呤或视黄酸诱导HL - 60细胞分化为粒细胞时，它们继续合成与未诱导细胞相似的糖肽。人外周血粒细胞主要合成大糖肽，而单核细胞和淋巴细胞主要合成小糖肽。这些结果表明，高分子量糖肽的合成是人类髓系白血病细胞系的一个特性，并且在整个髓系分化过程中持续存在。这些大糖肽合成的相应减少是单核细胞和巨噬细胞分化程序的一部分。
