Department of Microbiology, Goa University, Taleigao, India.
J Appl Microbiol. 2022 Mar;132(3):1900-1913. doi: 10.1111/jam.15309. Epub 2021 Oct 19.
To investigate the ability of Haloferax alexandrinus GUSF-1 (KF796625) to biosynthesize non-toxic elemental selenium (Se ) and check their capacity in in vitro crystal structure modulation of calcium oxalate, which are implicated in the development of renal calculi.
Haloferax alexandrinus GUSF-1 (KF796625) during growth in the presence of 5 mmol L of selenite formed insoluble brick-red particles. Se formed was monitored spectrophotometrically using a combination of two assays; the ascorbic acid reduction and sodium sulphide solubilization assay. After 168 h of growth, 2.89 mmol L of Se was formed from 4.9 mmol L of selenite. Absorption bands at 1.5, 11.2 and 12.5 keV in EDX spectroscopy confirmed that the brick-red particulate matter was Se . Furthermore, these selenium nanoparticles (SeNPs) were pentagonal in shape in transmission electron microscopy imaging. The peak positions in X-ray diffractogram at 2θ values of 23.40°, 29.66°, 41.26°, 43.68°, 45.24°, 51.62°, 55.93° and 61.47° and the relative intensities further confirmed the formation of Se . In vitro addition of 50 and 100 µg ml of these SeNPs to the mixture of sodium chloride, calcium chloride and sodium oxalate affected and modulated the shape and size of rectangular-shaped calcium oxalate crystals (average area of 1.23 ± 0.2 µm ) to smaller rectangular-shaped crystals (average area of 0.54 ± 0.2 µm ) and spherical-shaped crystals (average area 0.13 ± 0.005 µm ).
Haloferax alexandrinus GUSF-1 (KF796625) transformed selenite to Se pentagonal nanoforms that modulated in vitro the formation of crystal shape and size of calcium oxalate.
There are no reports on conversion of selenite to Se among the Haloferax genera, and this study involving the formation of pentagonal SeNPs with capacity to modulate the formation of calcium oxalate crystals in haloarchaea is recorded as the first report and of significance in pharmaceutical research related to formulations abetting urinary calculi.
研究亚历山大盐菌 GUSF-1（KF796625）合成无毒元素硒（Se）的能力，并检测其在体外调节草酸钙晶体结构方面的能力，这与肾结石的形成有关。
亚历山大盐菌 GUSF-1（KF796625）在亚硒酸盐存在的情况下生长时形成不溶性砖红色颗粒。使用两种组合的比色法（抗坏血酸还原和硫化钠溶解测定法）来监测形成的 Se。生长 168 小时后，从 4.9 mmol/L 的亚硒酸盐中形成了 2.89 mmol/L 的 Se。EDX 光谱中的 1.5、11.2 和 12.5 keV 处的吸收带证实砖红色颗粒物质是 Se。此外，透射电子显微镜成像显示这些硒纳米颗粒（SeNPs）呈五边形。X 射线衍射图谱中 2θ 值为 23.40°、29.66°、41.26°、43.68°、45.24°、51.62°、55.93°和 61.47°的峰位以及相对强度进一步证实了 Se 的形成。体外向氯化钠、氯化钙和草酸钠混合物中添加 50 和 100μg/ml 这些 SeNPs 会影响并调节矩形草酸钙晶体的形状和大小（平均面积为 1.23±0.2 µm），使其变为较小的矩形晶体（平均面积为 0.54±0.2 µm）和球形晶体（平均面积为 0.13±0.005 µm）。
亚历山大盐菌 GUSF-1（KF796625）将亚硒酸盐转化为五边形纳米硒，体外调节了草酸钙晶体的形成形状和大小。
在盐杆菌属中没有关于将亚硒酸盐转化为 Se 的报道，而本研究涉及到具有调节 haloarchaea 中草酸钙晶体形成能力的五边形 SeNPs 的形成，这是首次报道，在与促进尿液结石形成的制剂相关的药物研究方面具有重要意义。
