Zhao Chengyan, Song Qiusheng, Zhu Lin, Ma Haihong
School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, China.
J Biomater Sci Polym Ed. 2022 Feb;33(3):313-328. doi: 10.1080/09205063.2021.1987693. Epub 2021 Oct 21.
In the present study, a novel nanogel of HPC-PMAA/PpIX with thermo- and pH sensitive performance and its application in cancer photodynamic therapy is reported. HPC-PMAA/PpIX nanogels were prepared by free radical polymerization method with HPC as template, hydroxypropyl cellulose (HPC), methyl acrylic acid (MAA), protoporphyrin IX (PpIX) and ,'-methylene bisacrylamide (BIS) as raw materials. The as-prepared nanogels were characterized by Fourier transform infrared (FTIR), photoluminescence (PL) and UV-visible spectrophotometer (UV-vis), dynamic light scattering (DLS) and transmission electron microscopy (TEM). PL and UV-vis spectra demonstrate that PpIX is incorporated into HPC-PMAA by covalent bonds, and its aggregation is prevented. Moreover, the as-prepared nanogels can be dispersed in water over 1 week, significant singlet oxygen can be produced under irradiation of laser. With tumor cell of HepG2 as model cell, the nanogels are biocompatible with cell viability of >85% even at high concentrations of the PpIX . In addition, the HPC-PMAA/PpIX nanogels show photo-dependent toxicity in the concentration range of 10 µg/mL of PpIX, suggesting that HPC-PMAA/PpIX nanogels have potential for the treatment of photodynamic therapy (PDT).
在本研究中，报道了一种具有温度和pH敏感性的新型HPC-PMAA/PpIX纳米凝胶及其在癌症光动力治疗中的应用。以羟丙基纤维素（HPC）为模板，采用自由基聚合法，以羟丙基纤维素（HPC）、甲基丙烯酸（MAA）、原卟啉IX（PpIX）和N,N'-亚甲基双丙烯酰胺（BIS）为原料制备了HPC-PMAA/PpIX纳米凝胶。通过傅里叶变换红外光谱（FTIR）、光致发光光谱（PL）、紫外可见分光光度计（UV-vis）、动态光散射（DLS）和透射电子显微镜（TEM）对所制备的纳米凝胶进行了表征。PL和UV-vis光谱表明，PpIX通过共价键掺入HPC-PMAA中，防止了其聚集。此外，所制备的纳米凝胶在水中可分散超过1周，在激光照射下可产生大量单线态氧。以HepG2肿瘤细胞为模型细胞，即使在高浓度PpIX下，纳米凝胶与细胞的生物相容性良好，细胞活力>85%。此外，HPC-PMAA/PpIX纳米凝胶在PpIX浓度为10μg/mL范围内表现出光依赖性毒性，表明HPC-PMAA/PpIX纳米凝胶具有光动力治疗（PDT）的治疗潜力。
