Gao Jing, van Kleeff Paula J M, de Boer Mark H, Erban Alexander, Kopka Joachim, Hincha Dirk K, de Boer Albertus H
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China.
Department of Structural Biology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, Amsterdam, Netherlands.
Front Plant Sci. 2021 Sep 13;12:697324. doi: 10.3389/fpls.2021.697324. eCollection 2021.
Salinity is one of the major abiotic stresses that limits agricultural productivity worldwide. Many proteins with defined functions in salt stress adaptation are controlled through interactions with members of the 14-3-3 family. In the present study, we generated three 14-3-3 quadruple knockout mutants (qKOs: , and ) to study the role of six non-epsilon group 14-3-3 proteins for salt stress adaptation. The relative growth inhibition under 100 mM of NaCl stress was the same for wild-type (Wt) and qKOs, but the accumulation of Na in the shoots of was significantly lower than that in Wt. This difference correlated with the higher expression of the gene in . Considering the regulatory role of 14-3-3 proteins in metabolism and the effect of salt stress on metabolite accumulation, we analyzed the effect of a 24-h salt treatment on the root metabolome of nutrient solution-grown genotypes. The results indicated that the mutant had metabolome responses that were different from those of Wt. Notably, the reducing sugars, glucose and fructose, were lower in under control and salt stress. On the other hand, their phosphorylated forms, glucose-6P and fructose-6P, were lower under salt stress as compared to Wt. This study provided insight into the functions of the 14-3-3 proteins from non-epsilon group members. In summary, it was found that these proteins control ion homeostasis and metabolite composition under salt stress conditions and non-stressed conditions. The analyses of single, double, and triple mutants that modify subsets from the most effective qKO mutant () may also reveal the potential redundancy for the observed phenotypes.
盐度是限制全球农业生产力的主要非生物胁迫之一。许多在盐胁迫适应中具有明确功能的蛋白质是通过与14-3-3家族成员相互作用来调控的。在本研究中，我们构建了三个14-3-3四基因敲除突变体（qKOs： 、 和 ），以研究六种非ε组14-3-3蛋白在盐胁迫适应中的作用。在100 mM NaCl胁迫下，野生型（Wt）和qKOs的相对生长抑制相同，但 的地上部Na积累量显著低于Wt。这种差异与 中 基因的高表达相关。考虑到14-3-3蛋白在代谢中的调控作用以及盐胁迫对代谢物积累的影响，我们分析了24小时盐处理对营养液培养基因型根系代谢组的影响。结果表明， 突变体的代谢组反应与Wt不同。值得注意的是，在对照和盐胁迫下， 中的还原糖、葡萄糖和果糖含量较低。另一方面，与Wt相比，它们的磷酸化形式葡萄糖-6P和果糖-6P在盐胁迫下含量较低。本研究深入了解了非ε组成员的14-3-3蛋白的功能。总之，发现这些蛋白在盐胁迫条件和非胁迫条件下控制离子稳态和代谢物组成。对来自最有效的qKO突变体（ ）的单、双和三突变体的分析也可能揭示观察到的表型的潜在冗余性。
