Williams P P
Am J Vet Res. 1978 Mar;39(3):485-9.
Methods were developed for procuring phagocytically active macrophages from porcine lung with minimal damage to respiratory tissues. Procedures included anesthetizing, surgically introducing a T-shaped tracheal catheter, and repeatedly flushing the respiratory tract. Macrophages collected in this manner were characterized as to numbers, types, and phagocytic activity, nonselective lavage of the pulmonary airways of unstimulated and stimulated (evoking agent: thioglycolate medium) animals yielded 5 X 10(7) and 11 X 10(7) respiratory cells per pig, respectively. Because sufficient quantities (300 to 600 cells/test) of unstimulated cells were collected, stimulated cells contaminated with thioglycolate were not further tested. Morphologically, unstimulated macrophages were mainly spherical and mononucleated by variable in size, ranging from 9 to 30 micrometer. Culturally, macrophages adhered to plastic or glass surfaces and readily phagocytized fungal spores, staphylococci, and latex particles in an enrichment medium containing greater than or equal to 20% bovine fetal serum. Macrophages failed to replicate during a 3-week maintenance period. The data suggest that porcine phagocytes of the pulmonary system comprise a free-cell population that is a major surface-constitutive part of the luminal surface of the airways.
已开发出从猪肺中获取具有吞噬活性的巨噬细胞且对呼吸组织损伤最小的方法。步骤包括麻醉、手术插入T形气管导管以及反复冲洗呼吸道。对以这种方式收集的巨噬细胞的数量、类型和吞噬活性进行了表征，对未刺激和刺激（激发剂：巯基乙酸盐培养基）动物的肺气道进行非选择性灌洗，每头猪分别产生5×10⁷和11×10⁷个呼吸细胞。由于收集到了足够数量（每次测试300至600个细胞）的未刺激细胞，因此未对被巯基乙酸盐污染的刺激细胞进行进一步测试。在形态学上，未刺激的巨噬细胞主要呈球形且单核，大小不一，范围为9至30微米。在培养方面，巨噬细胞粘附于塑料或玻璃表面，并在含有大于或等于20%胎牛血清的富集培养基中容易吞噬真菌孢子、葡萄球菌和乳胶颗粒。巨噬细胞在3周的维持期内未能复制。数据表明，猪肺系统的吞噬细胞构成了一个游离细胞群体，是气道腔表面的主要表面组成部分。
