CRISPR Cas9-Mediated Selective Isothermal Amplification for Sensitive Detection of Rare Mutant Alleles.
作者信息
Chen Junman, Qiu Tian, Mauk Michael G, Fan Yaguang, Jiang Yong, Ying Jianming, Zhou Qinghua, Qiao Youlin, Bau Haim H, Song Jinzhao
机构信息
The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.
Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA, USA.
出版信息
Clin Chem. 2021 Nov 1;67(11):1569-1571. doi: 10.1093/clinchem/hvab163.
Abstract
摘要
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CUT-PCR: CRISPR-mediated, ultrasensitive detection of target DNA using PCR.CUT-PCR：利用PCR技术实现CRISPR介导的超灵敏靶标DNA检测。
Oncogene. 2017 Dec 7;36(49):6823-6829. doi: 10.1038/onc.2017.281. Epub 2017 Aug 28.
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Elimination of unaltered DNA in mixed clinical samples via nuclease-assisted minor-allele enrichment.通过核酸酶辅助的次要等位基因富集消除混合临床样本中未改变的DNA。
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