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Programmable endonuclease combined with isothermal polymerase amplification to selectively enrich for rare mutant allele fractions.可编程核酸内切酶与等温聚合酶扩增相结合以选择性富集稀有突变等位基因片段。
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Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics.使用嗜热栖热菌 Argonaute 高度特异性富集稀有核酸片段及其在癌症诊断中的应用。
Nucleic Acids Res. 2020 Feb 28;48(4):e19. doi: 10.1093/nar/gkz1165.
2
CUT-PCR: CRISPR-mediated, ultrasensitive detection of target DNA using PCR.CUT-PCR：利用PCR技术实现CRISPR介导的超灵敏靶标DNA检测。
Oncogene. 2017 Dec 7;36(49):6823-6829. doi: 10.1038/onc.2017.281. Epub 2017 Aug 28.
3
Elimination of unaltered DNA in mixed clinical samples via nuclease-assisted minor-allele enrichment.通过核酸酶辅助的次要等位基因富集消除混合临床样本中未改变的DNA。
Nucleic Acids Res. 2016 Nov 2;44(19):e146. doi: 10.1093/nar/gkw650. Epub 2016 Jul 18.
4
Depletion of Abundant Sequences by Hybridization (DASH): using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applications.通过杂交去除丰富序列（DASH）：利用Cas9在测序文库和分子计数应用中去除不需要的高丰度物种。
Genome Biol. 2016 Mar 4;17:41. doi: 10.1186/s13059-016-0904-5.
5
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.CRISPR RNA 引导的内切酶 Cas9 对 DNA 的检测。
Nature. 2014 Mar 6;507(7490):62-7. doi: 10.1038/nature13011. Epub 2014 Jan 29.

CRISPR Cas9-Mediated Selective Isothermal Amplification for Sensitive Detection of Rare Mutant Alleles.

作者信息

Chen Junman, Qiu Tian, Mauk Michael G, Fan Yaguang, Jiang Yong, Ying Jianming, Zhou Qinghua, Qiao Youlin, Bau Haim H, Song Jinzhao

机构信息

The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang, China.

Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA, USA.

出版信息

Clin Chem. 2021 Nov 1;67(11):1569-1571. doi: 10.1093/clinchem/hvab163.

DOI:10.1093/clinchem/hvab163
PMID:34590692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8561782/
Abstract
摘要