载溶菌酶的光交联明胶/胶原膜作为一种抗菌性角膜植入物。
Photo-crosslinked GelMA/collagen membrane loaded with lysozyme as an antibacterial corneal implant.
机构信息
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510006, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, PR China; Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China.
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510006, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, PR China; Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou 510005, PR China.
出版信息
Int J Biol Macromol. 2021 Nov 30;191:1006-1016. doi: 10.1016/j.ijbiomac.2021.09.144. Epub 2021 Sep 27.
Corneal transplantation is an effective treatment for corneal blindness. However, it brings risk factors for the occurrence of bacterial keratitis, which can affect the repair effect and even lead to transplantation failure. The difficulty in re-epithelialization is also a main problem faced by corneal transplantation. Herein, a collagen-GelMA composite membrane containing lysozyme (CGL) was developed as an antibacterial corneal implant to fill stromal defect and support re-epithelialization. Characterizations of physicochemical properties and in vitro biocompatibility revealed that the composite membranes have proper water content, light transmittance and mechanical strength as well as good biocompatibility. Particularly, the cell adhesion force and adhesion-related genes expression were evaluated and exhibited an improvement after the addition of GelMA. Furthermore, the formed CGL membrane could continuously release lysozyme and exhibited a bactericidal rate of 96% and 64% after 2 h and 72 h, respectively. The results demonstrated that this CGL membrane has promising application in corneal repair.
角膜移植是治疗角膜盲的有效手段。然而，它也带来了细菌角膜炎发生的风险因素，这可能会影响修复效果，甚至导致移植失败。角膜移植所面临的另一个主要问题是再上皮化的困难。本文中，开发了一种含有溶菌酶的胶原-明胶复合膜（CGL）作为一种抗菌性角膜植入物，以填充基质缺损并支持再上皮化。理化性质和体外生物相容性的表征表明，复合膜具有适当的含水量、透光率和机械强度以及良好的生物相容性。特别是，通过添加明胶，评估了细胞黏附力和黏附相关基因的表达，并显示出了改善。此外，形成的 CGL 膜能够持续释放溶菌酶，分别在 2 小时和 72 小时后表现出 96%和 64%的杀菌率。结果表明，这种 CGL 膜在角膜修复方面具有广阔的应用前景。
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