Long Yuyu, Ren Li, Wang Jiaming, Chen Min, Liu Yang, Liu Binqian, Wang Yingjun, Ge Jian
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2013 Feb;30(1):170-5.
In order to investigate the feasibility of the modified chitosan-gelatin crosslinked membrane (MC-Gel) and chitosan-gelatin crosslinked membrane (CS-Gel) to be a potential biomaterial for corneal regeneration, we evaluated their physicochemical properties and intraocular biocompatibility in this study. White light transmission and permeability of these membranes were detected. Results showed that white light transmission of both membranes was above 90% at 500 nm, which was similar to that of human cornea. The glucose, tryptophan and NaCl permeability of MC-Gel membrane and CS-Gel membrane was better than or similar to those of human cornea. The methylthiazol tetrazolium (MTT) assay was used to assess cell viability and proliferation. Also, interlamellar corneal transplantation was carried out to evaluate ophthalmic biocompatibility of MC-Gel membrane and CS-Gel membrane. Results indicated that MC-Gel membranes could support the proliferation of HCEC and displayed good intraocular biocompatibility when implanted into rabbits. No severe inflammatory reaction occurred after transplantation and the implanted MC-Gel membrane degraded completely 16 weeks post-operation. Due to its good physicochemical properties and biocompatibility, MC-Gel membrane could be a promising candidate material for corneal regeneration.
为了研究改性壳聚糖 - 明胶交联膜（MC - Gel）和壳聚糖 - 明胶交联膜（CS - Gel）作为角膜再生潜在生物材料的可行性，我们在本研究中评估了它们的物理化学性质和眼内生物相容性。检测了这些膜的白光透过率和通透性。结果表明，两种膜在500 nm处的白光透过率均高于90%，与人角膜相似。MC - Gel膜和CS - Gel膜的葡萄糖、色氨酸和氯化钠通透性优于或与人角膜相似。采用甲基噻唑四氮唑（MTT）法评估细胞活力和增殖情况。此外，进行层间角膜移植以评估MC - Gel膜和CS - Gel膜的眼内生物相容性。结果表明，MC - Gel膜能够支持人角膜内皮细胞（HCEC）的增殖，植入兔眼后显示出良好的眼内生物相容性。移植后未发生严重炎症反应，植入的MC - Gel膜在术后16周完全降解。由于其良好的物理化学性质和生物相容性，MC - Gel膜可能是角膜再生的一种有前景的候选材料。
