对 SARS-CoV-2 病毒进行全基因组测序，以评估在儿科医护人员中普遍戴口罩对病毒传播的影响。
Viral whole-genome sequencing to assess impact of universal masking on SARS-CoV-2 transmission among pediatric healthcare workers.
机构信息
Northwestern University Feinberg School of Medicine, Chicago, Illinois.
Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois.
出版信息
Infect Control Hosp Epidemiol. 2022 Oct;43(10):1408-1412. doi: 10.1017/ice.2021.415. Epub 2021 Oct 1.
OBJECTIVE
To identify the impact of universal masking on COVID-19 incidence and putative SARS-CoV-2 transmissions events among children's hospital healthcare workers (HCWs).
DESIGN
Quasi-experimental study.
SETTING
Single academic free-standing children's hospital.
METHODS
We performed whole-genome sequencing of SARS-CoV-2- PCR-positive samples collected from HCWs 3 weeks before and 6 weeks after implementing a universal masking policy. Phylogenetic analyses were performed to identify clusters of clonally related SARS-CoV-2 indicative of putative transmission events. We measured COVID-19 incidence, SARS-CoV-2 test positivity rates, and frequency of putative transmission events before and after the masking policy was implemented.
RESULTS
HCW COVID-19 incidence and test positivity declined from 14.3 to 4.3 cases per week, and from 18.4% to 9.0%, respectively. Putative transmission events were only identified prior to universal masking.
CONCLUSIONS
A universal masking policy was associated with reductions in HCW COVID-19 infections and occupational acquisition of SARS-CoV-2.
目的
确定普遍佩戴口罩对儿童医院医护人员（HCWs）COVID-19 发病率和假定 SARS-CoV-2 传播事件的影响。
设计
准实验研究。
设置
单家学术型独立儿童医院。
方法
我们对实施普遍佩戴口罩政策前 3 周和后 6 周内从 HCWs 采集的 SARS-CoV-2-PCR 阳性样本进行全基因组测序。进行系统发育分析以识别提示假定传播事件的具有克隆相关性的 SARS-CoV-2 聚类。我们在实施口罩政策前后测量了 COVID-19 发病率、SARS-CoV-2 检测阳性率和假定传播事件的频率。
结果
HCW COVID-19 发病率和检测阳性率从每周 14.3 例降至 4.3 例，从 18.4%降至 9.0%。假定的传播事件仅在普遍佩戴口罩之前被识别。
结论
普遍佩戴口罩政策与 HCW COVID-19 感染和 SARS-CoV-2 职业性获得的减少有关。
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