Di Santo Lionel N, Polgar Monica, Nies Storm, Hodgkiss Paul, Canning Courtney A, Wright Jessica W, Hamilton Jill A
Department of Biological Sciences, North Dakota State University, Fargo, ND, USA.
USDA Forest Service, Pacific Southwest Research Station, Davis, CA, USA.
AoB Plants. 2021 Sep 8;13(5):plab058. doi: 10.1093/aobpla/plab058. eCollection 2021 Oct.
Understanding the within- and among-population distribution of trait variation within seed collections may provide a means to approximate standing genetic variation and inform plant conservation. This study aimed to estimate population- and family-level seed trait variability for existing seed collections of Torrey pine (), and to use these data to guide sampling of future collections. We quantified variation in 14 seed morphological traits and seedling emergence within and among Torrey pine populations. Using a simulation-based approach, we used estimates of within-population variance to assess the number of maternal families required to capture 95 % of trait variation within each existing seed collection. Substantial structure was observed both within and among Torrey pine populations, with island and mainland seeds varying in seed size and seed coat thickness. Despite morphological differences, seedling emergence was similar across populations. Simulations revealed that 83 % and 71 % of all maternal families within island and mainland seed collections respectively needed to be resampled to capture 95 % of seed trait variation within existing collections. From a conservation perspective, our results indicate that to optimize genetic diversity captured in Torrey pine seed collections, maximizing the number of maternal families sampled within each population will be necessary.
了解种子收集品中性状变异在种群内和种群间的分布情况，可能为估算现存遗传变异以及为植物保护提供信息提供一种方法。本研究旨在估计现有火炬松种子收集品在种群和家系水平上的种子性状变异性，并利用这些数据指导未来收集品的采样。我们量化了火炬松种群内和种群间14种种子形态性状和幼苗出苗情况的变异。采用基于模拟的方法，我们利用种群内方差估计值来评估每个现有种子收集品中捕获95%性状变异所需的母系家系数目。在火炬松种群内和种群间均观察到显著的结构差异，岛屿和大陆的种子在种子大小和种皮厚度上有所不同。尽管形态存在差异，但各种群间的幼苗出苗情况相似。模拟结果显示，岛屿和大陆种子收集品中分别需要重新采样83%和71%的所有母系家系，才能捕获现有收集品中95%的种子性状变异。从保护的角度来看，我们的结果表明，为了优化火炬松种子收集品中捕获的遗传多样性，有必要在每个种群内最大化采样的母系家系数目。
