Charrier Guillaume, Burlett Régis, Gambetta Gregory, Delzon Sylvain, Domec Jean-Christophe, Beaujard François
Bordeaux Sciences Agro, Institut des Sciences de la Vigne et du Vin, Ecophysiologie et Génomique Fonctionnelle de la Vigne, UMR 1287, Villenave d'Ornon, France.
BIOGECO, INRA, Univ. Bordeaux, Cestas, France.
Bio Protoc. 2017 Oct 20;7(20):e2580. doi: 10.21769/BioProtoc.2580.
Xylem sap circulates under either positive or negative hydraulic pressure in plants. Negative hydraulic pressure (, tension) is the most common situation when transpiration is high, and several devices have been developed to quantify it accurately (, Scholander pressure chamber, psychrometers). However, a proper measurement of positive xylem sap pressures may be critical when pressure is generated by the root system, allowing vessels to be refilled. Here, we describe two different methods to monitor positive xylem bulk pressure: the pressure gauge which can only be set onto a rootstock or a side branch and the point pressure sensor, which can allow measurements from a functioning plant without detopping or cutting.
在植物中，木质部汁液在正水压或负水压下循环。当蒸腾作用强烈时，负水压（即张力）是最常见的情况，并且已经开发出了几种设备来精确量化它（如， Scholander压力室、湿度计）。然而，当根系产生压力使导管重新充满时，对木质部汁液正压力进行适当测量可能至关重要。在此，我们描述两种监测木质部整体正压力的不同方法：一种是只能安装在砧木或侧枝上的压力计，另一种是可以在不进行去顶或切割操作的情况下对正常生长的植物进行测量的点压力传感器。
