NLK激酶测定
NLK Kinase Assay.
作者信息
Moon Sungho, Kim Jiyoung, Jho Eek-Hoon
机构信息
Department of Life Science, University of Seoul, Seoul, Korea.
出版信息
Bio Protoc. 2017 Nov 5;7(21):e2593. doi: 10.21769/BioProtoc.2593.
Abstract
This protocol provides step by step instructions to perform an kinase assay for nemo-like kinase. In addition, this protocol also describes an efficient method using mild lysis buffer for expression and purification of Glutathione S-transferase (GST) fusion proteins.
摘要
本方案提供了进行尼莫样激酶激酶分析的逐步操作说明。此外，本方案还描述了一种使用温和裂解缓冲液表达和纯化谷胱甘肽S-转移酶（GST）融合蛋白的有效方法。
相似文献
1
NLK Kinase Assay.NLK激酶测定
Bio Protoc. 2017 Nov 5;7(21):e2593. doi: 10.21769/BioProtoc.2593.
2
Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.NLK介导的磷酸化作用会抑制YAP与14-3-3的相互作用，并促使其进入细胞核。
EMBO Rep. 2017 Jan;18(1):61-71. doi: 10.15252/embr.201642683. Epub 2016 Dec 15.
3
Two-step chromatographic purification of glutathione S-transferase-tagged human papillomavirus type 16 E6 protein and its application for serology.谷胱甘肽S-转移酶标记的人乳头瘤病毒16型E6蛋白的两步色谱纯化及其在血清学中的应用
Protein Expr Purif. 2017 Apr;132:19-26. doi: 10.1016/j.pep.2017.01.004. Epub 2017 Jan 9.
4
Lentivirus-based RNA silencing of Nemo-like kinase (NLK) inhibits the CAL 27 human adenosquamos carcinoma cells proliferation and blocks G0/G1 phase to S phase.慢病毒载体介导的 NLK 基因 RNA 干扰抑制 CAL 27 人颊鳞癌细胞增殖并阻滞细胞周期于 G0/G1 期。
Int J Med Sci. 2013 Aug 8;10(10):1301-6. doi: 10.7150/ijms.6607. eCollection 2013.
5
Nemo-like kinase (NLK) inhibits the progression of NSCLC via negatively modulating WNT signaling pathway.尼莫样激酶（NLK）通过负向调节WNT信号通路来抑制非小细胞肺癌的进展。
J Cell Biochem. 2014 Jan;115(1):81-92. doi: 10.1002/jcb.24635.
6
Expression of Nemo-like kinase was increased and negatively correlated with the expression of TCF4 in lung cancers.肺癌中Nemo样激酶的表达增加，且与TCF4的表达呈负相关。
Int J Clin Exp Pathol. 2015 Nov 1;8(11):15086-92. eCollection 2015.
7
Osmotic stress-induced phosphorylation by NLK at Ser128 activates YAP.由NLK在丝氨酸128位点进行的渗透应激诱导的磷酸化作用激活了YAP。
EMBO Rep. 2017 Jan;18(1):72-86. doi: 10.15252/embr.201642681. Epub 2016 Dec 15.
8
Overexpression of Nemo-like Kinase Promotes the Proliferation and Invasion of Lung Cancer Cells and Indicates Poor Prognosis.Nemo 样激酶过表达促进肺癌细胞的增殖和侵袭并预示不良预后。
Curr Cancer Drug Targets. 2019;19(8):674-680. doi: 10.2174/1568009618666181119150521.
9
Fbxw7 acts as an E3 ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation.Fbxw7作为一种E3泛素连接酶，靶向c-Myb以进行Nemo样激酶（NLK）诱导的降解。
J Biol Chem. 2008 Nov 7;283(45):30540-8. doi: 10.1074/jbc.M804340200. Epub 2008 Sep 2.
10
The emerging role of Nemo-like kinase (NLK) in the regulation of cancers.尼莫样激酶（NLK）在癌症调控中的新作用。
Tumour Biol. 2015 Dec;36(12):9147-52. doi: 10.1007/s13277-015-4159-7. Epub 2015 Oct 1.
引用本文的文献
1
Genome-wide CRISPR screens identify PKMYT1 as a therapeutic target in pancreatic ductal adenocarcinoma.全基因组 CRISPR 筛选鉴定 PKMYT1 为胰腺导管腺癌的治疗靶点。
EMBO Mol Med. 2024 May;16(5):1115-1142. doi: 10.1038/s44321-024-00060-y. Epub 2024 Apr 3.
本文引用的文献
1
Phosphorylation by NLK inhibits YAP-14-3-3-interactions and induces its nuclear localization.NLK介导的磷酸化作用会抑制YAP与14-3-3的相互作用，并促使其进入细胞核。
EMBO Rep. 2017 Jan;18(1):61-71. doi: 10.15252/embr.201642683. Epub 2016 Dec 15.
2
Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex.Nemo-like 激酶通过干扰 Notch 活性转录复合物的形成来抑制 Notch 信号通路。
Nat Cell Biol. 2010 Mar;12(3):278-85. doi: 10.1038/ncb2028. Epub 2010 Jan 31.
3
Regulation of FOXO1 by TAK1-Nemo-like kinase pathway.TAK1-Nemo 样激酶通路对 FOXO1 的调控。
J Biol Chem. 2010 Mar 12;285(11):8122-9. doi: 10.1074/jbc.M110.101824. Epub 2010 Jan 8.
4
The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF.TAK1-NLK-MAPK相关通路拮抗β-连环蛋白与转录因子TCF之间的信号传导。
Nature. 1999 Jun 24;399(6738):798-802. doi: 10.1038/21674.
5
WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans.WRM-1激活LIT-1蛋白激酶，以在秀丽隐杆线虫中传导前后极性信号。
Cell. 1999 Jun 11;97(6):717-26. doi: 10.1016/s0092-8674(00)80784-9.
6
Nlk is a murine protein kinase related to Erk/MAP kinases and localized in the nucleus.Nlk是一种与Erk/MAP激酶相关的小鼠蛋白激酶，定位于细胞核。
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):963-8. doi: 10.1073/pnas.95.3.963.
7
Rotation of photoreceptor clusters in the developing Drosophila eye requires the nemo gene.在发育中的果蝇眼睛中，光感受器簇的旋转需要nemo基因。
Cell. 1994 Jul 15;78(1):125-36. doi: 10.1016/0092-8674(94)90579-7.
Zotero 插件