State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, Shandong, China.
Academy of Advanced Interdisciplinary Studies, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, Shandong, China.
Anal Bioanal Chem. 2021 Nov;413(26):6639-6647. doi: 10.1007/s00216-021-03630-y. Epub 2021 Sep 30.
Herein, a novel, convenient, and highly selective electrochemical sensor for determination of nitrite based on a polythiophene-derivative film-modified glassy carbon electrode (GCE) was established. In this work, 2,5-di-thiophen-3-yl-thiazolo[5,4-d]thiazole (DTT), a novel thiophene derivative, was synthesized and used to form an original and excellent polymer film (PolyDTTF) on GCE through one-step electropolymerization for the first time. The modified electrodes were characterized by electron microscopy (SEM), Fourier transform infra-red spectroscopy (FT-IR), UV-visible spectra, Raman spectroscopy, and electrochemical technologies, in which the electrochemical sensor based on PolyDTTF was successfully constructed and demonstrated a significant electrocatalytic effect on nitrite. The influence of pH value, electrodeposition scanning times, scanning speed, and potential on the electrochemical behavior of nitrite were investigated in detail. Furthermore, the nitrite sensor exhibits excellent responses proportional to nitrite concentrations (R = 0.9972) over a concentration range of 5.5 × 10 ~ 3.5 × 10 M with a detection limit (LOD) of 2 nM, and has extremely good anti-interference ability for nitrite detection. This proposed sensor can be used to detect nitrite in actual samples, opening the possibility for applications in the food industry and environmental analysis.
本文建立了一种基于聚噻吩衍生物修饰玻碳电极的新型、简便、高选择性电化学传感器，用于测定亚硝酸盐。在这项工作中，首次合成了一种新型噻吩衍生物 2,5-二噻吩-3-基噻唑[5,4-d]噻唑（DTT），并通过一步电化学聚合在 GCE 上形成原始且优异的聚合物膜（PolyDTTF）。修饰电极通过电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、紫外可见光谱、拉曼光谱和电化学技术进行了表征，其中成功构建了基于 PolyDTTF 的电化学传感器，并对亚硝酸盐表现出显著的电催化作用。详细研究了 pH 值、电沉积扫描次数、扫描速度和电位对亚硝酸盐电化学行为的影响。此外，该亚硝酸盐传感器在 5.5×103.5×10M 的浓度范围内对亚硝酸盐浓度表现出优异的线性响应（R=0.9972），检测限（LOD）低至 2 nM，对亚硝酸盐检测具有极强的抗干扰能力。该提出的传感器可用于检测实际样品中的亚硝酸盐，为食品工业和环境分析中的应用开辟了可能性。
Zotero 插件