State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, Shandong, China.
Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, College of Chemistry and Food Science, Yulin Normal University, Yulin, 537000, Guangxi, China.
Anal Bioanal Chem. 2022 Jul;414(17):4837-4847. doi: 10.1007/s00216-022-04106-3. Epub 2022 May 6.
Herein, we fabricated a label-free ECL immunosensor for aflatoxin B (AFB) detection. In this system, a small organic aggregation-induced electrochemiluminescence luminophore, 2,5-di-tetraphenylethylene-ylthiazolo [5,4-d] thiazole, was designed, named TPETTZ. Polyaniline-wrapped TiO nanoparticles (PANI/TiO NPs) complex was synthesized through one-step in situ oxidation polymerization of aniline, and performed excellent electrical conductivity and abundant amino groups. As an ECL accelerator, TiO nanoparticles (TiO NPs) promoted the oxidation of tri-n-propylamine (TPA) to generate more TPA; in addition, it also acted as a donor to improve the ECL intensity of TPETTZ (acceptor) through electrochemiluminescence resonance energy transfer (ECL-RET). Encouraged by the above, under the existence of TPA, TPETTZ displayed a strong and continuously stable ECL signal due to the introduction of PANI/TiO NPs. Therefore, the immunosensor was constructed for AFB detection based on the quenching effect of target on the ECL signal, and a linearly decreasing ECL signal was obtained as the increasement of AFB in the range of 75 fg/mL to 100 ng/mL, with a lower detection limit of 27.5 fg/mL. Moreover, the as-prepared sensing platform performed a satisfactory anti-interference, stability, and reproducibility, and appeared a good accuracy in walnut sample analysis, presenting a promising application in the future.
在此，我们构建了一种用于检测黄曲霉毒素 B（AFB）的无标记电化学发光（ECL）免疫传感器。在该体系中，设计并合成了一种小分子有机聚集诱导的电致化学发光（ECL）发光体，即 2,5-二（四苯乙烯基）噻唑并[5,4-d]噻唑（TPETTZ）。通过苯胺的一步原位氧化聚合，合成了具有优异导电性和丰富氨基的聚苯胺包裹的 TiO2纳米粒子（PANI/TiO2NPs）复合物。作为 ECL 加速剂，TiO2纳米粒子（TiO2NPs）促进了三正丙胺（TPA）的氧化，从而产生更多的 TPA；此外，它还通过电化学发光共振能量转移（ECL-RET）充当供体，提高了 TPETTZ（受体）的 ECL 强度。受此启发，在 TPA 的存在下，由于引入了 PANI/TiO2NPs，TPETTZ 显示出强且持续稳定的 ECL 信号。因此，基于目标物对 ECL 信号的猝灭效应，构建了用于 AFB 检测的免疫传感器，并获得了线性降低的 ECL 信号，随着 AFB 浓度在 75 fg/mL 至 100 ng/mL 范围内的增加，其检测下限为 27.5 fg/mL。此外，所制备的传感平台具有令人满意的抗干扰性、稳定性和重现性，在核桃样品分析中表现出良好的准确性，具有广阔的应用前景。
