原发性嗜酸性粒细胞性结肠炎中的肥大细胞数量显著高于继发性组织嗜酸性粒细胞增多症和正常对照：这可能与发病机制有关。

Mast Cell Numbers in Primary Eosinophilic Colitis are Significantly Higher than in Secondary Tissue Eosinophilia and Normal Control: a Possible Link to Pathogenesis.

作者信息

Awad Heyam, Sfaira Ammar, Abu Osba Yousef, Shahin Mohammad, Al-Asa'd Yousef, Isbeih Nooredeen, Hayagneh Wasan, Shomaf Maha

机构信息

Histopathology, Microbiology and Forensic Medicine Department, University of Jordan, Amman, Jordan.

出版信息

Iran J Immunol. 2021 Sep;18(3):220-229. doi: 10.22034/iji.2021.88577.1881.

DOI:10.22034/iji.2021.88577.1881

PMID:34596587

Abstract

BACKGROUND

Primary Eosinophilic Colitis (PEC) is one of the rare eosinophilic gastrointestinal diseases with a poorly understood pathogenesis. Eosinophilic esophagitis (EE) is the most common and best-understood disease in this category. Activated mast cells (MCs) have a role to play in the tissue damage in EE. It is not known if PEC shares this mechanism.

OBJECTIVE

This cross-sectional study aimed to investigate the number of MCs in PEC and to compare them with cases of secondary colonic tissue eosinophilia (TE) and normal colon.

METHODS

The study included 19 PEC cases, 47 cases of secondary tissue eosinophilia and 50 normal colon tissues. Histopathological slides of all cases were reviewed to confirm the diagnosis and count the number of eosinophils. Glass slides for all cases were stained for C-kit (CD117) to highlight and count the MCs.

RESULTS

The mean number of the MCs in normal controls was 9.7 MCs per HPF (SD= 4.6). The mean number of MCs in the PEC cases was 26.5 (SD=7.1) which was significantly higher than the normal counts (p-value <0.000). The mean number of MCs in the secondary TE group was 18.0 (SD=7.1), which was significantly higher than normal controls; p-value <0.000. Comparing MC counts in PEC and secondary TE also revealed a significant difference with a p-value of < 0.000.

CONCLUSION

MCs in PEC are significantly higher than those in secondary TE and normal controls. This suggests the role of the MCs in the pathogenesis of Primary Eosinophilic Colitis.

摘要

背景

原发性嗜酸性粒细胞性结肠炎（PEC）是一种罕见的嗜酸性粒细胞性胃肠道疾病，其发病机制尚不清楚。嗜酸性粒细胞性食管炎（EE）是此类疾病中最常见且了解最深入的疾病。活化的肥大细胞（MCs）在EE的组织损伤中起作用。尚不清楚PEC是否具有相同机制。

目的

本横断面研究旨在调查PEC中MCs的数量，并将其与继发性结肠组织嗜酸性粒细胞增多症（TE）病例和正常结肠进行比较。

方法

该研究纳入了19例PEC病例、47例继发性组织嗜酸性粒细胞增多症病例和50例正常结肠组织。对所有病例的组织病理学切片进行复查以确诊并计数嗜酸性粒细胞数量。对所有病例的玻片进行C-kit（CD117）染色，以突出显示并计数MCs。

结果

正常对照组中MCs的平均数量为每高倍视野9.7个（标准差=4.6）。PEC病例中MCs的平均数量为26.5个（标准差=7.1），显著高于正常计数（p值<0.000）。继发性TE组中MCs的平均数量为18.0个（标准差=7.1），显著高于正常对照组；p值<0.000。比较PEC和继发性TE中的MC计数也显示出显著差异，p值<0.000。