Department of Chemistry, Faculty of Science, Al-Balqa Applied University, Al-Salt, Jordan.
Methods Mol Biol. 2022;2371:19-29. doi: 10.1007/978-1-0716-1689-5_2.
Solid-phase peptide synthesis (SPPS) is the method of choice that enables the access to a library of synthetic bioactive peptides. Cyclization due to the presence of disulfide bridges is of great importance for certain proteins and peptides since it increases their stability against proteolysis by constraining the conformations of these peptides and proteins. Here we describe the solid-phase peptide cyclization by on-resin strategy represented in the synthesis of peptides containing two disulfide bridges. The presence and/or the absence of free SH groups can be investigated by Ellman's test.
固相肽合成 (SPPS) 是一种能够获得合成生物活性肽库的方法。由于二硫键的存在，环化对于某些蛋白质和肽非常重要，因为它通过限制这些肽和蛋白质的构象来增加它们对蛋白水解的稳定性。在这里，我们描述了在含有两个二硫键的肽合成中通过树脂上策略进行的固相肽环化。可以通过 Ellman 的测试来研究游离 SH 基团的存在和/或不存在。
