Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
J Wound Care. 2021 Sep 2;30(Sup9a):XIVi-XIViii. doi: 10.12968/jowc.2021.30.Sup9a.XIV.
Otomycosis is a fungal infection of the external auditory canal caused mainly by the genus , an industrially important fungus, is a member of section . In this report, we present a case of otomycosis due to in a 43-year-old female patient. We performed a partial PCR-sequencing of β-tubulin and calmodulin genes to identify the isolate to the species level. Further, we determined the in vitro susceptibility of the isolate to nystatin, clotrimazole and itraconazole according to the Clinical & Laboratory Standards Institute (CLSI) M38-A2 protocol. Accordingly, the minimum inhibitory concentrations of clotrimazole, nystatin and itraconazole were 0.25μg/mL, 0.5μg/mL and 1μg/mL, respectively. This is the first report of clinically relevant isolation of identified by a molecular technique as a causative agent of otomycosis.
耳真菌病是一种由真菌引起的外耳道感染，主要由 属引起，该属真菌是一种具有工业重要性的真菌，属于 节。在本报告中，我们报告了一例由 引起的 43 岁女性耳真菌病病例。我们对β-微管蛋白和钙调蛋白基因进行了部分 PCR 测序，以将分离株鉴定到种的水平。此外，我们根据临床和实验室标准协会（CLSI）M38-A2 方案，测定了分离株对制霉菌素、克霉唑和伊曲康唑的体外敏感性。因此，克霉唑、制霉菌素和伊曲康唑的最小抑菌浓度分别为 0.25μg/mL、0.5μg/mL 和 1μg/mL。这是首例通过分子技术鉴定为耳真菌病致病因子的 临床相关分离株的报告。
