Iyer Maalavika S, Wang Fu-Ming, Jayapalan Ramana Ramya, Veeramani Subha, Rajangam Ilangovan
National Centre for Nanoscience and Nanotechnology, University of Madras, Chennai, 600 025, Tamil Nadu, India.
Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, 106, Taiwan, ROC.
Anal Biochem. 2021 Dec 1;634:114393. doi: 10.1016/j.ab.2021.114393. Epub 2021 Sep 28.
The electrochemical performance of dual layer immunosensor has been studied by employing reduced Graphene oxide (rGO) and its nanocomposites with Carbon Nanofibers (CNFs) and Carbon Nanotubes (CNTs) as supporting matrix for the detection of CA125. The immunosensor determination was based on the formation of antibody - antigen immunocomplex, a decrement in the current response was observed in accordance with the concentration of antigen. Better performance exhibited by rGO/CNF in terms of linearity (99%) and sensitivity 0.65 μA (μg mL) can be attributed to its conductivity and surface area. The nanocomposite are employed in the detection of CA125 with linear working range of 10-32 × 10 μg mL, the limit of detection is found to be 0.28 pg mL rGO nanocomposite with CNT (rGO/CNT) is studied as transducer material. rGO/CNT exhibited better linearity when compared to rGO due to its good conductivity. Thus, graphene nanocomposite transducer materials have vital application in detection of oncomarkers.
通过使用还原氧化石墨烯（rGO）及其与碳纳米纤维（CNF）和碳纳米管（CNT）的纳米复合材料作为检测CA125的支撑基质，研究了双层免疫传感器的电化学性能。免疫传感器的测定基于抗体 - 抗原免疫复合物的形成，观察到电流响应随着抗原浓度的增加而降低。rGO/CNF在线性度（99%）和灵敏度0.65 μA/（μg mL）方面表现出更好的性能，这可归因于其导电性和表面积。该纳米复合材料用于检测CA125，线性工作范围为10 - 32×10 μg mL，检测限为0.28 pg mL，研究了含CNT的rGO纳米复合材料（rGO/CNT）作为换能器材料。由于其良好的导电性，rGO/CNT与rGO相比表现出更好的线性度。因此，石墨烯纳米复合换能器材料在肿瘤标志物检测中具有重要应用。
