Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Biosens Bioelectron. 2020 Apr 1;153:112029. doi: 10.1016/j.bios.2020.112029. Epub 2020 Jan 16.
In the current study, we report on the design and development of a novel electrochemical immunosensor for the detection of cancer antigen 125 (CA125) oncomarker. Polyamidoamine/gold nanoparticles (PAMAM/AuNPs) were used to increase the conductivity and enhance the number of antibodies (Abs) immobilized on the electrode surface. Three-dimensional reduced graphene oxide-multiwall carbon nanotubes (3DrGO-MWCNTs) were used to modify the glassy carbon electrode to improve the electrode conductivity and specific surface area. Ab and toluidine blue attached to O-succinyl-chitosan-magnetic nanoparticles (Suc-CS@MNPs) as a tracer. The poor solubility of chitosan (CS) was improved by succinic anhydride using a novel modification method. Under optimum condition, the developed immunosensor exhibited a wide linear range (0.0005-75 U/mL) and an excellent limit of detection around 6 μU/mL. The reliability of the engineered immunosensor in detecting CA125 was verified by standard addition recovery method, which was further compared to enzyme-linked immunosorbent assay (ELISA). The proposed immunosensor exhibited excellent stability, high selectivity and sensitivity, and good reproducibility. Based on the great performance of the engineered immunosensor, it is proposed as a robust and reliable diagnostic tool for the detection of CA125 in the clinic.
在本研究中，我们报告了一种用于检测癌症抗原 125（CA125）肿瘤标志物的新型电化学免疫传感器的设计和开发。聚酰胺-胺/金纳米粒子（PAMAM/AuNPs）用于增加导电性并增强电极表面固定的抗体（Abs）数量。三维还原氧化石墨烯-多壁碳纳米管（3DrGO-MWCNTs）用于修饰玻碳电极以提高电极导电性和比表面积。Ab 和甲苯胺蓝连接到 O-琥珀酰壳聚糖-磁性纳米粒子（Suc-CS@MNPs）作为示踪剂。通过使用新型修饰方法，用琥珀酸酐提高了壳聚糖（CS）的溶解度。在最佳条件下，开发的免疫传感器表现出宽线性范围（0.0005-75 U/mL）和优异的检测限约为 6 μU/mL。通过标准添加回收方法验证了工程免疫传感器在检测 CA125 方面的可靠性，该方法进一步与酶联免疫吸附测定（ELISA）进行了比较。所提出的免疫传感器表现出出色的稳定性、高选择性和灵敏度以及良好的重现性。基于工程免疫传感器的优异性能，建议将其作为一种用于临床检测 CA125 的强大而可靠的诊断工具。
