Cold Spring Harb Protoc. 2021 Oct 1;2021(10):2021/10/pdb.prot103309. doi: 10.1101/pdb.prot103309.
Ascitic fluid (also called ascites) is an intraperitoneal fluid extracted from mice that have developed a peritoneal tumor. For antibody production, the tumor is induced by injecting hybridoma cells into the peritoneum, which serves as a growth chamber for the cells. The hybridoma cells grow to high densities and continue to secrete the antibody of interest, thus creating a high-titered solution of antibodies for collection. A single mouse may yield as much as 10 mL of ascitic fluid or as little as 1 mL per batch. Antibody concentrations will typically be between 1 and 10 mg/mL. The most common problem encountered in storing ascites is contamination of these solutions with bacteria or fungi. This can be prevented by the addition of sodium azide.
腹水（也称为腹水）是从患有腹膜肿瘤的小鼠中提取的腹腔内液体。为了产生抗体，通过将杂交瘤细胞注入腹膜中来诱导肿瘤，腹膜充当细胞的生长室。杂交瘤细胞生长到高密度并继续分泌感兴趣的抗体，从而产生高滴度的抗体溶液以供收集。一只小鼠可能产生多达 10 毫升的腹水，也可能每批只有 1 毫升。抗体浓度通常在 1 至 10 毫克/毫升之间。在储存腹水中最常见的问题是这些溶液被细菌或真菌污染。可以通过添加叠氮化钠来防止这种情况。
